ELISA Kit DIY Materials for Defensin Alpha 3, Neutrophil Specific (DEFa3)
HNP3; DEF3; HNP-2; Neutrophil defensin 2
- Product No.KSE135Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.47-30ng/mL
- Applicable Sensitivity0.21ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1260
For more details, please contact local distributors! US$ 2100 US$ 3780 US$ 7350 US$ 12600
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Defensin Alpha 3, Neutrophil Specific (DEFa3). No significant cross-reactivity or interference between Defensin Alpha 3, Neutrophil Specific (DEFa3) and analogues was observed
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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