Coating Buffer

Instruction manual

First Edition (Revised on April, 2016)

PRODUCT INFORMATION

Coating Buffer can help the capture antibody binding on the inner-surface of wells in ELISA plate, for later conjugation of antibody and target antigen. An efficient and stable antibody coating buffer, which can enhance the stability of antibody, and increase the affinity of antibody and inner-surface of wells, has been developed by Cloud-Clone Corp..

REAGENTS AND MATERIALS PROVIDED

Name	Quantity	Components
Coating Buffer	1 bottle of powder	Na2CO3, NaHCO3, et al.

NOTE: * The working solutions should be clear.

*If working solution is prepared, please store at 2-8ºC, and use up within one week.

STORAGE AND PERIOD OF VALIDITY

It is valid for one year at room temperature. Please make solution fresh before the experiment.

Application

Prepare a working solution by diluting the Coating Buffer with deionized or distilled water before the experiment. 1 bottle can be used for preparing 220ml working solution.

IMPORTANT NOTES

1. Please avoid microbial contamination.

2. If precipitate has been found, please place the coating buffer in water bath with 37℃ till completely dissolution. Appropriate care should be exercised when using these reagents, including gloves, glasses, lab coats, and good laboratory procedures.