Packages (Simulation)

Polyclonal Antibody to Defensin Alpha 3, Neutrophil Specific (DEFa3)

Image (I)

DAB staining on IHC-P; Samples: Human Glioma Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human DEFa3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human DEFa3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human DEFa3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Pancreas Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human DEFa3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human DEFa3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human DEFa3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 30µg/ml Rabbit Anti-Human DEFa3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue
Primary Ab: 30µg/ml Rabbit Anti-Human DEFa3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant DEFa3, Human.
Western Blot; Sample: Lane1: Porcine Lung lysate; Lane2: Porcine Spleen lysate; Lane3: Porcine Pancreas lysate
Primary Ab: 2µg/ml Rabbit Anti-Human DEFa3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Defensin-Alpha-3--Neutrophil-Specific-(DEFa3)-PAE135Hu02.pdf

Certificate

Polyclonal Antibody to Defensin Alpha 3, Neutrophil Specific (DEFa3)

HNP3; DEF3; HNP-2; Neutrophil defensin 2

Product No.PAE135Hu02
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPE135Hu01-Recombinant Defensin Alpha 3, Neutrophil Specific (DEFa3)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreSus scrofa; Porcine (Pig)
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against DEFa3. It has been selected for its ability to recognize DEFa3 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-3µg/mL
Immunohistochemistry: 5-30µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPE135Hu01	Recombinant Defensin Alpha 3, Neutrophil Specific (DEFa3)	Positive Control; Immunogen; SDS-PAGE; WB.
RPE135Hu02	Recombinant Defensin Alpha 3, Neutrophil Specific (DEFa3)	Positive Control; Immunogen; SDS-PAGE; WB.
PAE135Hu01	Polyclonal Antibody to Defensin Alpha 3, Neutrophil Specific (DEFa3)	WB; IHC; ICC; IP.
PAE135Hu02	Polyclonal Antibody to Defensin Alpha 3, Neutrophil Specific (DEFa3)	WB,IHC
LAE135Hu81	FITC-Linked Polyclonal Antibody to Defensin Alpha 3, Neutrophil Specific (DEFa3)	WB; IHC; ICC; IF.
LAE135Hu71	Biotin-Linked Polyclonal Antibody to Defensin Alpha 3, Neutrophil Specific (DEFa3)	WB; IHC; ICC.
SEE135Hu	ELISA Kit for Defensin Alpha 3, Neutrophil Specific (DEFa3)	Enzyme-linked immunosorbent assay for Antigen Detection.
SCE135Hu	CLIA Kit for Defensin Alpha 3, Neutrophil Specific (DEFa3)	Chemiluminescent immunoassay for Antigen Detection.
LME135Hu	Multiplex Assay Kit for Defensin Alpha 3, Neutrophil Specific (DEFa3) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
KSE135Hu01	ELISA Kit DIY Materials for Defensin Alpha 3, Neutrophil Specific (DEFa3)	Main materials for "Do It (ELISA Kit) Yourself".