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ELISA Kit DIY Materials for Heat Shock 70kDa Protein 5 (HSPA5)

MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78

  • Product No.KSC343Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.781-50ng/mL
  • Applicable Sensitivity0.32ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1368  US$ 2280  US$ 4104  US$ 7980  US$ 13680 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 5 (HSPA5). No significant cross-reactivity or interference between Heat Shock 70kDa Protein 5 (HSPA5) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPC343Ra01 Recombinant Heat Shock 70kDa Protein 5 (HSPA5) Positive Control; Immunogen; SDS-PAGE; WB.
PAC343Ra01 Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) WB; IHC; ICC; IP.
MAC343Ra21 Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) WB; IHC; ICC; IP.
SEC343Ra ELISA Kit for Heat Shock 70kDa Protein 5 (HSPA5) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC343Ra Multiplex Assay Kit for Heat Shock 70kDa Protein 5 (HSPA5) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC343Ra01 ELISA Kit DIY Materials for Heat Shock 70kDa Protein 5 (HSPA5) Main materials for "Do It (ELISA Kit) Yourself".