Packages (Simulation)
![ELISA Kit for Heat Shock 70kDa Protein 5 (HSPA5)](/upload/static/product/SE.jpg)
Reagent Preparation
![ELISA Kit for Heat Shock 70kDa Protein 5 (HSPA5)](/upload/static/reagent/TB.jpg)
Image (I)
Image (II)
Certificate
![Certificate](/upload/static/ISO13485.jpg)
![Certificate](/upload/static/ce-1.jpg)
ELISA Kit for Heat Shock 70kDa Protein 5 (HSPA5)
MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78
- Product No.SEC343Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.312-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.115ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 532
For more details, please contact local distributors! US$ 760 US$ 3420 US$ 6460 US$ 53200
Specificity
This assay has high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 5 (HSPA5).
No significant cross-reactivity or interference between Heat Shock 70kDa Protein 5 (HSPA5) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Heat Shock 70kDa Protein 5 (HSPA5) and the recovery rates were calculated by comparing the measured value to the expected amount of Heat Shock 70kDa Protein 5 (HSPA5) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 85-98 | 89 |
EDTA plasma(n=5) | 80-103 | 86 |
heparin plasma(n=5) | 89-99 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heat Shock 70kDa Protein 5 (HSPA5) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 89-97% | 90-104% | 82-104% | 78-101% |
EDTA plasma(n=5) | 78-96% | 90-97% | 93-105% | 80-101% |
heparin plasma(n=5) | 81-92% | 80-92% | 78-97% | 79-92% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
GIVEAWAYS
INCREMENT SERVICES
-
Single-component Reagents of Assay Kit
-
Lysis Buffer Specific for ELISA / CLIA
-
Quality Control of Kit
-
ELISA Kit Customized Service
-
Disease Model Customized Service
-
Serums Customized Service
-
TGFB1 Activation Reagent
-
Real Time PCR Experimental Service
-
Streptavidin
-
Fast blue Protein Stain solution
-
Single-component Reagents of FLIA Kit
-
Streptavidin-Agarose Beads
Magazine | Citations |
41 | Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes. PubMed: 20522592 |
pharmacological research | Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors Pubmed: 31048034 |
EPILEPSY & BEHAVIOR | Fenfluramine acts as a positive modulator of sigma-1 receptors Pubmed: 32169824 |
Journal of Oncology | Using Serological Proteome Analysis to Identify and Evaluate Anti-GRP78 Autoantibody as Biomarker in the Detection of Gastric Cancer 33381181 |
Heliyon | Glucose-regulated protein 78 (GRP78) in renal cell carcinoma: A novel biomarker for predicting tumor behavior |
Medicine (Baltimore) | Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients 34414940 |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
RPC343Ra01 | Recombinant Heat Shock 70kDa Protein 5 (HSPA5) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC343Ra01 | Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) | WB; IHC; ICC; IP. |
MAC343Ra21 | Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) | WB; IHC; ICC; IP. |
SEC343Ra | ELISA Kit for Heat Shock 70kDa Protein 5 (HSPA5) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC343Ra | Multiplex Assay Kit for Heat Shock 70kDa Protein 5 (HSPA5) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSC343Ra01 | ELISA Kit DIY Materials for Heat Shock 70kDa Protein 5 (HSPA5) | Main materials for "Do It (ELISA Kit) Yourself". |