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Recombinant Heat Shock 70kDa Protein 5 (HSPA5)

MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78

  • Product No.RPC343Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationCytoplasm
  • Predicted Molecular Mass24.6kDa
  • Accurate Molecular Mass25kDa(Analysis of differences refer to the manual)
  • Residues & TagsGln260~Glu469 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point8.4
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 140  US$ 350  US$ 700  US$ 2100  US$ 5250 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
41 Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes. PubMed: 20522592
pharmacological research Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors Pubmed: 31048034
EPILEPSY & BEHAVIOR Fenfluramine acts as a positive modulator of sigma-1 receptors Pubmed: 32169824
Journal of Oncology Using Serological Proteome Analysis to Identify and Evaluate Anti-GRP78 Autoantibody as Biomarker in the Detection of Gastric Cancer 33381181
Heliyon Glucose-regulated protein 78 (GRP78) in renal cell carcinoma: A novel biomarker for predicting tumor behavior
Medicine (Baltimore) Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients 34414940
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPC343Ra01 Recombinant Heat Shock 70kDa Protein 5 (HSPA5) Positive Control; Immunogen; SDS-PAGE; WB.
PAC343Ra01 Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) WB; IHC
MAC343Ra21 Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5) WB; IHC; ICC; IP.
SEC343Ra ELISA Kit for Heat Shock 70kDa Protein 5 (HSPA5) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC343Ra Multiplex Assay Kit for Heat Shock 70kDa Protein 5 (HSPA5) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC343Ra01 ELISA Kit DIY Materials for Heat Shock 70kDa Protein 5 (HSPA5) Main materials for "Do It (ELISA Kit) Yourself".