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ELISA Kit DIY Materials for Afamin (AFM)

ALB2; ALBA; ALF; A-ALB; Alpha-Albumin

  • Product No.KSC284Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range3.12-200ng/mL
  • Applicable Sensitivity1.48ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1135  US$ 1891  US$ 3404  US$ 6619  US$ 11346 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Afamin (AFM). No significant cross-reactivity or interference between Afamin (AFM) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Proteomics 2D-DIGE analysis of sera from transgenic mouse models reveals novel candidate protein biomarkers for human gastric cancer ScienceDirect: S1874391912004964
Clin Chim Acta Afamin — A pleiotropic glycoprotein involved in various disease states PubMed: 25892677
Nutrients Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … Pubmed:30037068
Biomarkers in Medicine Urine afamin and afamin–creatinine ratio as biomarkers for kidney injury Pubmed: 30433819
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC284Mu01 Recombinant Afamin (AFM) Positive Control; Immunogen; SDS-PAGE; WB.
APC284Mu01 Active Afamin (AFM) Cell culture; Activity Assays.
PAC284Mu01 Polyclonal Antibody to Afamin (AFM) WB; IHC; ICC; IP.
LAC284Mu81 FITC-Linked Polyclonal Antibody to Afamin (AFM) WB; IHC; ICC; IF.
LAC284Mu71 Biotin-Linked Polyclonal Antibody to Afamin (AFM) WB; IHC; ICC.
KSC284Mu01 ELISA Kit DIY Materials for Afamin (AFM) Main materials for "Do It (ELISA Kit) Yourself".