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Western Blot; Sample: Mouse Serum
Primary Ab: 0.2μg/ml Biotin-Linked Polyclonal Antibody to Afamin (AFM)
Second Ab: 0.2μg/ml HRP-Labeled Streptavidin (Catalog: IS089)
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Biotin-Linked Polyclonal Antibody to Afamin (AFM)
ALB2; ALBA; ALF; A-ALB; Alpha-Albumin
- Product No.LAC284Mu71
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourceAntibody labeling
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies. - Downloadn/a
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 119
US$ 277
US$ 396
US$ 990
US$ 3960
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against AFM. It has been selected for its ability to recognize AFM in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Journal of Proteomics | 2D-DIGE analysis of sera from transgenic mouse models reveals novel candidate protein biomarkers for human gastric cancer ScienceDirect: S1874391912004964 |
| Clin Chim Acta | Afamin — A pleiotropic glycoprotein involved in various disease states PubMed: 25892677 |
| Nutrients | Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … Pubmed:30037068 |
| Biomarkers in Medicine | Urine afamin and afamin–creatinine ratio as biomarkers for kidney injury Pubmed: 30433819 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC284Mu01 | Recombinant Afamin (AFM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC284Mu01 | Active Afamin (AFM) | Cell culture; Activity Assays. |
| PAC284Mu01 | Polyclonal Antibody to Afamin (AFM) | WB; IHC; ICC; IP. |
| LAC284Mu81 | FITC-Linked Polyclonal Antibody to Afamin (AFM) | WB; IHC; ICC; IF. |
| LAC284Mu71 | Biotin-Linked Polyclonal Antibody to Afamin (AFM) | WB; IHC; ICC. |
| KSC284Mu01 | ELISA Kit DIY Materials for Afamin (AFM) | Main materials for "Do It (ELISA Kit) Yourself". |