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Active Afamin (AFM)

ALB2; ALBA; ALF; A-ALB; Alpha-Albumin

ACTIVITY TEST

Afamin (AFM, also known as Alpha -Albumin), a bioactive protein, is expressed in the liver and secreted into the bloodstream. Human afamin is a 87 kDa protein which is encoded by the fourth member of the human albumin gene family. Mouse AFM shares 66% aa sequence identity with Full-length human AFM. This protein can bind and transport vitamin E across the blood-brain barrier in body fluids under conditions where the lipoprotein system is not sufficient, it also can act as a carrier for hydrophobic molecules in body fluids. Essential for the solubility and activity of lipidated Wnt family members. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse AFM and recombinant human WNT3A. Briefly, AFM was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to WNT3A-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-AFM pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse AFM and recombinant human WNT3A was shown in Figure 1, the EC50 for this effect is 0.01 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Proteomics 2D-DIGE analysis of sera from transgenic mouse models reveals novel candidate protein biomarkers for human gastric cancer ScienceDirect: S1874391912004964
Clin Chim Acta Afamin — A pleiotropic glycoprotein involved in various disease states PubMed: 25892677
Nutrients Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … Pubmed:30037068
Biomarkers in Medicine Urine afamin and afamin–creatinine ratio as biomarkers for kidney injury Pubmed: 30433819
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC284Mu01 Recombinant Afamin (AFM) Positive Control; Immunogen; SDS-PAGE; WB.
APC284Mu01 Active Afamin (AFM) Cell culture; Activity Assays.
PAC284Mu01 Polyclonal Antibody to Afamin (AFM) WB; IHC; ICC; IP.
LAC284Mu81 FITC-Linked Polyclonal Antibody to Afamin (AFM) WB; IHC; ICC; IF.
LAC284Mu71 Biotin-Linked Polyclonal Antibody to Afamin (AFM) WB; IHC; ICC.
KSC284Mu01 ELISA Kit DIY Materials for Afamin (AFM) Main materials for "Do It (ELISA Kit) Yourself".