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ELISA Kit DIY Materials for Syndecan 1 (SDC1)
CD138; SDC; SYND1; Syndecan Proteoglycan 1
- Product No.KSB966Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range1.56-100ng/mL
- Applicable Sensitivity0.63ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1197
US$ 1995
US$ 3591
US$ 6983
US$ 11970
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Syndecan 1 (SDC1). No significant cross-reactivity or interference between Syndecan 1 (SDC1) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Molecular and Cellular Biochemistry | Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarction SpringerLink: a201w3l18k257462 |
| Inflammation | Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI Rats Springer: Source |
| Atherosclerosis. | Damage of the endothelial glycocalyx in chronic kidney disease Pubmed:24727235 |
| Journal of Trauma and Acute Care Surgery | Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injury Pubmed:25058256 |
| Shock | Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance Pubmed:25692257 |
| Mediators Inflamm | Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding PubMed: 26199464 |
| Transplantation | Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury. PubMed: 26371596 |
| Clinical and Experimental Nephrology | Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury pubmed:27405618 |
| Biomarkers in Medicine | Endothelial glycocalyx layer shedding following lung resection pubmed:27643669 |
| Danish National RESEARCH DATABASE | Resuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats 10.1089 |
| Journal of Neurotrauma | Resuscitation with pooled and pathogen-reduced plasma attenuates the increase in brain water content following traumatic brain injury and hemorrhagic shock in rats the rat. doi/10.1089/neu.2016.4574 |
| British Journal of Anaesthesia | Volume kinetics of Ringer's lactate solution in acute inflammatory disease 10.1016:j.bja.2018.04.023 |
| Arteriosclerosis, Thrombosis, and Vascular Biology | MPO (myeloperoxidase) reduces endothelial glycocalyx thickness dependent on its cationic charge Pubmed:29903730 |
| Shock | Plasma resuscitation improved survival in a cecal ligation and puncture rat model of sepsis Pubmed:28591008 |
| Shock | Experimental models of endotheliopathy: impact of shock severity Pubmed:28697004 |
| Journal of Trauma and Acute Care Surgery | Effects of propranolol and clonidine on brain edema, blood-brain barrier permeability, and endothelial glycocalyx disruption after fluid percussion brain injury in the rat Pubmed:28930945 |
| The Endothelial Glycocalyx in the Peritoneal Microcirculation of Rats with Chronic Kidney Failure Exposed to Dialysis Solutions 173015_06.pdf | |
| Journal of Surgical Research | Plasma ameliorates endothelial dysfunction in burn injury Doi: 10.1016/j.jss.2018.08.027 |
| Journal of Anesthesia | Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke model Pubmed: 30374889 |
| Anaesthesia | Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass Pubmed: 30687934 |
| Journal of Cardiothoracic and Vascular Anesthesial | Microvascular alterations during cardiac surgery using a heparin or phosphorylcholine coated circuit Pubmed: 31787433 |
| Hepatobiliary & Pancreatic Diseases International | Increased syndecan-1 and glypican-3 predict poor perinatal outcome and treatment resistance in intrahepatic cholestasis: Syndecan-1, glypican-3 and ICP Pubmed: 31919038 |
| Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study | |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Newly Developed Recombinant Antithrombin Protects the Endothelial Glycocalyx in an Endotoxin-Induced Rat Model of Sepsis 33375342 |
| Microvasc Res | Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency 34119534 |
| J Gerontol A Biol Sci Med Sci | The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo … 34423832 |
| Shock | Inhalation of 2% hydrogen improves survival rate and attenuates shedding of vascular endothelial glycocalyx in rats with heat stroke 34524269 |
| Exp Anim | Dexmedetomidine suppresses serum syndecan-1 elevation and improves survival in a rat hemorrhagic shock model Pubmed:35110424 |
| Journal of Emergency Medicine | Resuscitation of hemorrhagic shock using normal saline does not damage the glycocalyx in the immediate resuscitation phase |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| EPB966Hu62 | Eukaryotic Syndecan 1 (SDC1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB966Hu01 | Recombinant Syndecan 1 (SDC1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB966Hu61 | Eukaryotic Syndecan 1 (SDC1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB966Hu02 | Recombinant Syndecan 1 (SDC1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB966Hu03 | Recombinant Syndecan 1 (SDC1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB966Hu01 | Polyclonal Antibody to Syndecan 1 (SDC1) | WB; IHC; ICC/IF |
| FAB966Hu01 | Anti-Syndecan 1 (SDC1) Polyclonal Antibody | Flow cytometry. |
| FAB966Hu41 | PE-Linked Anti-Syndecan 1 (SDC1) Polyclonal Antibody | Flow cytometry. |
| FAB966Hu51 | APC-Linked Anti-Syndecan 1 (SDC1) Polyclonal Antibody | Flow cytometry. |
| FAB966Hu81 | FITC-Linked Anti-Syndecan 1 (SDC1) Polyclonal Antibody | FCM |
| MAB966Hu21 | Monoclonal Antibody to Syndecan 1 (SDC1) | WB; IHC |
| MAB966Hu23 | Monoclonal Antibody to Syndecan 1 (SDC1) | WB |
| MAB966Hu22 | Monoclonal Antibody to Syndecan 1 (SDC1) | WB |
| MAB966Hu24 | Monoclonal Antibody to Syndecan 1 (SDC1) | WB |
| MAB966Hu25 | Monoclonal Antibody to Syndecan 1 (SDC1) | WB |
| RAB966Hu21 | Recombinant Antibody to Syndecan 1 (SDC1) | WB; IF; ICC; IHC; IP; FCM. |
| MAB966Hu27 | Monoclonal Antibody to Syndecan 1 (SDC1) | IHC |
| MAB966Hu29 | Monoclonal Antibody to Syndecan 1 (SDC1) | WB; IHC; ICC/IF |
| SEB966Hu | ELISA Kit for Syndecan 1 (SDC1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEB966Hu | High Sensitive ELISA Kit for Syndecan 1 (SDC1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB966Hu | Multiplex Assay Kit for Syndecan 1 (SDC1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSB966Hu01 | ELISA Kit DIY Materials for Syndecan 1 (SDC1) | Main materials for "Do It (ELISA Kit) Yourself". |