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ELISA Kit DIY Materials for Insulin Degrading Enzyme (IDE)

Insulysin; Insulin Protease; Abeta-degrading protease; Insulinase

  • Product No.KSB897Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.59ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1197  US$ 1995  US$ 3591  US$ 6983  US$ 11970 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Insulin Degrading Enzyme (IDE). No significant cross-reactivity or interference between Insulin Degrading Enzyme (IDE) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
J Neurochem Reduction PubMed: 26442993
African Journal of Pharmacy and Pharmacology Peptide hydroxamate derivatives as regulators for insulin receptor signaling and its degradation by zinc metalloprotease in diabetic rats article-full-text-pdf
Biomedicine & Pharmacotherapy Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats. pubmed:27930980
Biological Trace Element Research Nigella sativa Oil and Chromium Picolinate Ameliorate Fructose-Induced Hyperinsulinemia by Enhancing Insulin Signaling and Suppressing Insulin-Degrading Enzyme in Male Rats 10.1007/s12011-017-1167-z
Neuroscience The Protective Effects of IGF-I against β-Amyloid-related Downregulation of Hippocampal Somatostatinergic System Involve Activation of Akt and Protein Kinase A Pubmed:29406271
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB897Hu01 Recombinant Insulin Degrading Enzyme (IDE) Positive Control; Immunogen; SDS-PAGE; WB.
APB897Hu01 Active Insulin Degrading Enzyme (IDE) Cell culture; Activity Assays.
PAB897Hu01 Polyclonal Antibody to Insulin Degrading Enzyme (IDE) WB; IHC
MAB897Hu21 Monoclonal Antibody to Insulin Degrading Enzyme (IDE) WB; IHC; ICC; IP.
MAB897Hu22 Monoclonal Antibody to Insulin Degrading Enzyme (IDE) WB; IHC; ICC; IP.
SEB897Hu ELISA Kit for Insulin Degrading Enzyme (IDE) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB897Hu Multiplex Assay Kit for Insulin Degrading Enzyme (IDE) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB897Hu01 ELISA Kit DIY Materials for Insulin Degrading Enzyme (IDE) Main materials for "Do It(ELISA Kit) Yourself"