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Insulin Degrading Enzyme (IDE)

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Insulysin; Insulin Protease; Abeta-degrading protease; Insulinase

Insulin Degrading Enzyme (IDE)
CCC B897
HGNC 5381
MGI 96412
PubMed 12634421
RGD 2861
UniProt P14735
Wiki IDE

Insulin Degrading Enzyme (IDE) is a large zinc-binding protease of the M16A metalloprotease subfamily known to cleave multiple short polypeptides that vary considerably in sequence. IDE was first identified by its ability to degrade the B chain of the hormone insulin. This activity was observed over fifty years ago, though the enzyme specifically responsible for B chain cleavage was identified more recently. This discovery revealed considerable amino acid sequence homology between IDE and the previously characterized bacterial protease pitrilysin, suggesting a common proteolytic mechanism. IDE, which migrates at 110 kDa during gel electrophoresis under denaturing conditions, has since been shown to have additional substrates, including the signaling peptides glucagon, TGF alpha, and beta-endorphin.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB897Hu01 Recombinant Insulin Degrading Enzyme (IDE) Positive Control; Immunogen; SDS-PAGE; WB.
APB897Hu01 Active Insulin Degrading Enzyme (IDE) Cell culture; Activity Assays.
Antibodies PAB897Hu01 Polyclonal Antibody to Insulin Degrading Enzyme (IDE) WB; IHC
MAB897Hu22 Monoclonal Antibody to Insulin Degrading Enzyme (IDE) WB; IHC; ICC; IP.
MAB897Hu21 Monoclonal Antibody to Insulin Degrading Enzyme (IDE) WB; IHC; ICC; IP.
Assay Kits SEB897Hu ELISA Kit for Insulin Degrading Enzyme (IDE) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB897Mu01 Recombinant Insulin Degrading Enzyme (IDE) Positive Control; Immunogen; SDS-PAGE; WB.
RPB897Mu02 Recombinant Insulin Degrading Enzyme (IDE) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB897Mu01 Polyclonal Antibody to Insulin Degrading Enzyme (IDE) WB; IHC; ICC; IP.
PAB897Mu02 Polyclonal Antibody to Insulin Degrading Enzyme (IDE) WB; IHC; ICC; IP.
Assay Kits SEB897Mu ELISA Kit for Insulin Degrading Enzyme (IDE) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB897Ra01 Recombinant Insulin Degrading Enzyme (IDE) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB897Ra01 Polyclonal Antibody to Insulin Degrading Enzyme (IDE) WB; IHC; ICC; IP.
MAB897Ra21 Monoclonal Antibody to Insulin Degrading Enzyme (IDE) WB; IHC; ICC; IP.
Assay Kits SEB897Ra ELISA Kit for Insulin Degrading Enzyme (IDE) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III."Science 242:1415-1418(1988) [PubMed] [Europe PMC] [Abstract]
  2. "Insulin-degrading enzyme: stable expression of the human complementary DNA, characterization of its protein product, and chromosomal mapping of the human and mouse genes."Mol. Endocrinol. 4:1125-1135(1990) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and comparative analysis of human chromosome 10." Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Neurons regulate extracellular levels of amyloid beta-protein via proteolysis by insulin-degrading enzyme."J. Neurosci. 20:1657-1665(2000) [PubMed] [Europe PMC] [Abstract]
  7. "Insulin degrading enzyme is a cellular receptor mediating varicella-zoster virus infection and cell-to-cell spread."Cell 127:305-316(2006) [PubMed] [Europe PMC] [Abstract]
  8. "The amino terminus of varicella-zoster virus (VZV) glycoprotein E is required for binding to insulin-degrading enzyme, a VZV receptor."J. Virol. 81:8525-8532(2007) [PubMed] [Europe PMC] [Abstract]
  9. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  10. "Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism."Nature 443:870-874(2006) [PubMed] [Europe PMC] [Abstract]
  11. "Structure of substrate-free human insulin-degrading enzyme (IDE) and biophysical analysis of ATP-induced conformational switch of IDE."J. Biol. Chem. 282:25453-25463(2007) [PubMed] [Europe PMC] [Abstract]
  12. "Molecular bases for the recognition of short peptide substrates and cysteine-directed modifications of human insulin-degrading enzyme."Biochemistry 47:12822-12834(2008) [PubMed] [Europe PMC] [Abstract]