Packages (Simulation)

Polyclonal Antibody to Insulin Degrading Enzyme (IDE)

Image (I)

DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human IDE Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human IDE Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IDE Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Testis Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IDE Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Liver Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IDE Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IDE Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant IDE, Human.
Figure. Western Blot; Sample: Human HepG2 Cells.
Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: IDE knockout HepG2 cell lysate;
Predicted MW: 118,54kd
Observed MW: 100kd
Primary Ab: 1µg/ml Rabbit Anti-Human IDE Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Cerebrum lysate
Primary Ab: 0.01µg/ml Rabbit Anti-Human IDE Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: K562 cell lysate
Primary Ab: 0.4µg/ml Rabbit Anti-Human IDE Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Cerebrum lysate
Primary Ab: 0.01µg/ml Rabbit Anti-Human IDE Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Quality Guarantee

Instruction Manual

Polyclonal-Antibody-to-Insulin-Degrading-Enzyme-(IDE)-PAB897Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Insulin Degrading Enzyme (IDE)

Insulysin; Insulin Protease; Abeta-degrading protease; Insulinase

Product No.PAB897Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB897Hu01-Recombinant Insulin Degrading Enzyme (IDE)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against IDE. It has been selected for its ability to recognize IDE in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
J Neurochem	Reduction PubMed: 26442993
African Journal of Pharmacy and Pharmacology	Peptide hydroxamate derivatives as regulators for insulin receptor signaling and its degradation by zinc metalloprotease in diabetic rats article-full-text-pdf
Biomedicine & Pharmacotherapy	Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats. pubmed:27930980
Biological Trace Element Research	Nigella sativa Oil and Chromium Picolinate Ameliorate Fructose-Induced Hyperinsulinemia by Enhancing Insulin Signaling and Suppressing Insulin-Degrading Enzyme in Male Rats 10.1007/s12011-017-1167-z
Neuroscience	The Protective Effects of IGF-I against β-Amyloid-related Downregulation of Hippocampal Somatostatinergic System Involve Activation of Akt and Protein Kinase A Pubmed:29406271

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB897Hu01	Recombinant Insulin Degrading Enzyme (IDE)	Positive Control; Immunogen; SDS-PAGE; WB.
APB897Hu01	Active Insulin Degrading Enzyme (IDE)	Cell culture; Activity Assays.
PAB897Hu01	Polyclonal Antibody to Insulin Degrading Enzyme (IDE)	WB; IHC
MAB897Hu21	Monoclonal Antibody to Insulin Degrading Enzyme (IDE)	WB; IHC; ICC; IP.
MAB897Hu22	Monoclonal Antibody to Insulin Degrading Enzyme (IDE)	WB; IHC; ICC; IP.
SEB897Hu	ELISA Kit for Insulin Degrading Enzyme (IDE)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB897Hu	Multiplex Assay Kit for Insulin Degrading Enzyme (IDE) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
KSB897Hu01	ELISA Kit DIY Materials for Insulin Degrading Enzyme (IDE)	Main materials for "Do It(ELISA Kit) Yourself"