ELISA Kit DIY Materials for Transglutaminase 2 (TGM2)
tTG; TG2; C Polypeptide,Protein-Glutamine-Gamma-Glutamyltransferase; Transglutaminase 2, Tissue; Transglutaminase C; Transglutaminase H
- Product No.KSB830Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.625-40ng/mL
- Applicable Sensitivity0.227ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1197
For more details, please contact local distributors! US$ 1995 US$ 3591 US$ 6983 US$ 11970
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Transglutaminase 2, Tissue (TGM2). No significant cross-reactivity or interference between Transglutaminase 2, Tissue (TGM2) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Magazine | Citations |
Connective Tissue Research | Tissue transglutaminase is involved in mechanical load–induced osteogenic differentiation of human ligamentum flavum cells Pubmed:27115725 |
American Journal of Respiratory Cell and Molecular Biology | ECM Crosslinking Enhances Fibroblast Growth and Protects Against Matrix Proteolysis in Lung Fibrosis. pubmed:29053339 |
3 Biotech | Metformin attenuates TGF‑β1‑induced pulmonary fibrosis through inhibition of transglutaminase 2 and subsequent TGF‑β pathways Pubmed: 32550106 |
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