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Active Transglutaminase 2 (TGM2)

tTG; TG2; C Polypeptide,Protein-Glutamine-Gamma-Glutamyltransferase; Transglutaminase 2, Tissue; Transglutaminase C; Transglutaminase H

ACTIVITY TEST

Transglutaminase 2 (TGM2), encoded by the TGM2 gene, is belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions. In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP and has intrinsic kinase activity. TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases. The activity of recombinant human TGM2 is measured by its ability to cleave a synthetic peptide Benzyloxycarbonyl-Gln-Gly and NH2OH in the assay buffer 200 mM MES, 10 mM DTT, 10 mM CaCl2, 100 mM Hydroxylamine Hydrochloride, pH 6.0. The rhTGM2 is diluted to 12.5 ug/ml in assay buffer. Loading into a clear well plate 50 µL of 12.5 ug/mL rhTGM2 and start the reaction by adding 50 µL of 100 mM substrate, with a substrate blank containing 50 µL assay buffer, 50 µL substrate, and no rhTGM2. Incubated at 37 °C for 2 hours and stop the reaction with 400 ul stop solution of 0.37 M FeCl3, 0.67 M HCl，0.2 M Trichloroacetic Acid. Centrifuge at 2000 rpm for 2 minutes and then load 200 ul of the supernatant into a plate and read at 525 nm (absorbance) in endpoint mode. The specific activity of recombinant human TGM2 is > 800 pmol/min/µg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Connective Tissue Research Tissue transglutaminase is involved in mechanical load–induced osteogenic differentiation of human ligamentum flavum cells Pubmed:27115725
American Journal of Respiratory Cell and Molecular Biology ECM Crosslinking Enhances Fibroblast Growth and Protects Against Matrix Proteolysis in Lung Fibrosis. pubmed:29053339
3 Biotech Metformin attenuates TGF‑β1‑induced pulmonary fibrosis through inhibition of transglutaminase 2 and subsequent TGF‑β pathways Pubmed: 32550106
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