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ELISA Kit DIY Materials for Nephrin (NPHN)

NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor

  • Product No.KSA937Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleUrine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.124ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Nephrin (NPHN). No significant cross-reactivity or interference between Nephrin (NPHN) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Yonsei medical journal The Level of Serum and Urinary Nephrin in Normal Pregnancy and Pregnancy with Subsequent Preeclampsia. pubmed:28120572
Experimental and molecular pathology Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. pubmed:29246788
British Journal of Haematology  Renal protection by atorvastatin in a murine model of sickle cell nephropathy Pubmed:29527679
Journal of Clinical Medicine Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney Disease Pubmed: 32961903
Disease Markers Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study 34707724
Biomed Rep Early biomarkers of nephrotoxicity associated with the use of anti-VEGF drugs Pubmed:35620307
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA937Ra01 Recombinant Nephrin (NPHN) Positive Control; Immunogen; SDS-PAGE; WB.
PAA937Ra01 Polyclonal Antibody to Nephrin (NPHN) WB; IHC; ICC; IP.
MAA937Ra21 Monoclonal Antibody to Nephrin (NPHN) WB; IHC; ICC; IP.
SEA937Ra ELISA Kit for Nephrin (NPHN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA937Ra Multiplex Assay Kit for Nephrin (NPHN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA937Ra01 ELISA Kit DIY Materials for Nephrin (NPHN) Main materials for "Do It (ELISA Kit) Yourself".