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Recombinant Nephrin (NPHN)

NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor

  • Product No.RPA937Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Predicted Molecular Mass38.4kDa
  • Accurate Molecular Mass41kDa(Analysis of differences refer to the manual)
  • Residues & TagsPro39~Gly106 with N-terminal His and GST Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 97%
  • Isoelectric Point9.8
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 167  US$ 418  US$ 836  US$ 2508  US$ 6270 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Yonsei medical journal The Level of Serum and Urinary Nephrin in Normal Pregnancy and Pregnancy with Subsequent Preeclampsia. pubmed:28120572
Experimental and molecular pathology Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. pubmed:29246788
British Journal of Haematology  Renal protection by atorvastatin in a murine model of sickle cell nephropathy Pubmed:29527679
Journal of Clinical Medicine Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney Disease Pubmed: 32961903
Disease Markers Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study 34707724
Biomed Rep Early biomarkers of nephrotoxicity associated with the use of anti-VEGF drugs Pubmed:35620307
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA937Ra01 Recombinant Nephrin (NPHN) Positive Control; Immunogen; SDS-PAGE; WB.
PAA937Ra01 Polyclonal Antibody to Nephrin (NPHN) WB; IHC; ICC; IP.
MAA937Ra21 Monoclonal Antibody to Nephrin (NPHN) WB; IHC; ICC; IP.
SEA937Ra ELISA Kit for Nephrin (NPHN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA937Ra Multiplex Assay Kit for Nephrin (NPHN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA937Ra01 ELISA Kit DIY Materials for Nephrin (NPHN) Main materials for "Do It (ELISA Kit) Yourself".