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ELISA Kit DIY Materials for Parathyroid Hormone (PTH)

iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin

  • Product No.KSA866Ra11
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1,000pg/mL
  • Applicable Sensitivity4.43pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • DownloadInstruction Manual
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Parathyroid Hormone (PTH). No significant cross-reactivity or interference between Parathyroid Hormone (PTH) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Biological trace element research Elevation of PTH and PTHrp Induced by Excessive Fluoride in Rats on a Calcium-deficient Diet PubMed: 19915804
Basic nutritional investigation Effects of duodenal redox status on calcium absorption and related genes expression in high-fat diet–fed mice ScienceDirect: S0899900709004808
The Korean Journal of Nutrition Effects of a Low Calcium Diet and Oxalate Intake on Calcium Deposits in Soft Tissues and Bone Metabolism in Ovariectomized Rats KoreaMed: source
Acta Physiologica Hungarica Whole body vibration is a safe exercise training method and induces no impaired alterations on rat plasma parameters Pubmed: 22173025
Toxicology and Industrial Health The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat PubMed: 22782709
Indian Journal of Experimental Biology Effect of consumption of fatty acids, calcium, vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats. PubMed: 22439438
Plos one Is Gastrectomy-Induced High Turnover of Bone with Hyperosteoidosis and Increase of Mineralization a Typical Osteomalacia? PubMed: PMC3679169
Nutr Hosp. Effect of the “protein diet” and bone tissue. Pubmed: 24483972
Clinical Cancer Research Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivo Aacrjournals: Source
Nutricion Hospitalaria Effect of the “protein diet” and bone tissue Aulamedica: Source
Academic Journal Effect of whole body vibration on healthy rat plasma parameters Ebscohost: Source
J Proteome Res. Biomarkers identified by urinary metabonomics for noninvasive diagnosis of nutritional rickets Pubmed:25051233
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer PubMed: 26266370
J Nutr Sci Vitaminol (Tokyo) The Impact of Different Amounts of Calcium Intake on Bone Mass and Arterial Calcification in Ovariectomized Rats PubMed: 26639847
The Korean Society of Pharmacognosy Effects of Oryza sativa L. Aleurone Layer Extract on Bone Mineral Density and Bone-related Markers in the Ovariectomized Rat Article: Hksobf_2015_V46N2_167
J Bone Miner Res Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082
7 Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. pubmed:28218908
PLOS ONE Prostaglandin-E2 Mediated Increase in Calcium and Phosphate Excretion in a Mouse Model ofDistal Nephron Salt Wasting. pubmed:27442254
International Journal of Advanced Research in Biological Sciences Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat 10.22192/ijarbs.2017.04.04.018
American Journal of Physiology-Renal Physiology Chronic nicotine exposure reduces klotho expression and triggers different renal and hemodynamic responses in -haploinsufficient mice Pubmed:29363324
Tropical Animal Health and Production Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolism Pubmed:29931604
International Immunopharmacology CD8+ T lymphocytes enhance the anabolic effect of intermittent parathyroid hormone on cementoblasts Pubmed: 31679847
ENDOCRINOLOGY A New Therapeutic Approach Using a Calcilytic (AXT914) for Post-Surgical Hypoparathyroidism in Female Rats Pubmed: 32852547
Kidney International Calciprotein particles regulate fibroblast growth factor-23 expression in osteoblasts.
PREVENTIVE AND/OR THERAPEUTIC AGENT FOR OSTEOGENESIS IMPERFECTA AND OTHER DISEASES
Oxid Med Cell Longev Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats 34373766
Gene Therapy Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR1A vector 34599290
Cells Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury Pubmed:35456008
African Health Sciences Fibroblast Growth Factor 23 (FGF 23) and intact parathyroid hormone (iPTH) as markers of mineral bone disease among Nigerians with non-diabetic kidney disease
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APA866Ra01 Active Parathyroid Hormone (PTH) Cell culture; Activity Assays.
RPA866Ra01 Recombinant Parathyroid Hormone (PTH) Positive Control; Immunogen; SDS-PAGE; WB.
CPA866Ra11 BSA Conjugated Parathyroid Hormone (PTH) Immunogen; SDS-PAGE; WB.
CPA866Ra21 OVA Conjugated Parathyroid Hormone (PTH) Immunogen; SDS-PAGE; WB.
PAA866Ra08 Polyclonal Antibody to Parathyroid Hormone (PTH) WB; IHC; ICC; IP.
PAA866Ra01 Polyclonal Antibody to Parathyroid Hormone (PTH) WB; IHC; ICC; IP.
MAA866Ra21 Monoclonal Antibody to Parathyroid Hormone (PTH) WB; IHC; ICC; IP.
CEA866Ra ELISA Kit for Parathyroid Hormone (PTH) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA866Ra CLIA Kit for Parathyroid Hormone (PTH) Chemiluminescent immunoassay for Antigen Detection.
LMA866Ra Multiplex Assay Kit for Parathyroid Hormone (PTH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA866Ra11 ELISA Kit DIY Materials for Parathyroid Hormone (PTH) Main materials for "Do It (ELISA Kit) Yourself".