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Active Parathyroid Hormone (PTH)

iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin

ACTIVITY TEST

PTH (Parathyroid hormone) is a hormone secreted by the parathyroid glands that is important in bone remodeling. As reported, osteoblast-like cell lines, such as ROS 17/2.8, UMR106, SaOS, U2OS, MG63, that exhibit PTHR1, respond with increased proliferation to PTH. Rat PTH shares similarities with human PTH in amino acid sequence with the identity of 71.3%. Thus, a proliferation assay of rat recombinant PTH was conducted using U2OS cells. Briefly, U2OS cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of PTH. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of U2OS cells after incubation with PTH for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant PTH for 48h. The result was shown in Figure 2. It was obvious that PTH increased cell viability of U2OS cells.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Biological trace element research Elevation of PTH and PTHrp Induced by Excessive Fluoride in Rats on a Calcium-deficient Diet PubMed: 19915804
Basic nutritional investigation Effects of duodenal redox status on calcium absorption and related genes expression in high-fat diet–fed mice ScienceDirect: S0899900709004808
The Korean Journal of Nutrition Effects of a Low Calcium Diet and Oxalate Intake on Calcium Deposits in Soft Tissues and Bone Metabolism in Ovariectomized Rats KoreaMed: source
Acta Physiologica Hungarica Whole body vibration is a safe exercise training method and induces no impaired alterations on rat plasma parameters Pubmed: 22173025
Toxicology and Industrial Health The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat PubMed: 22782709
Indian Journal of Experimental Biology Effect of consumption of fatty acids, calcium, vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats. PubMed: 22439438
Plos one Is Gastrectomy-Induced High Turnover of Bone with Hyperosteoidosis and Increase of Mineralization a Typical Osteomalacia? PubMed: PMC3679169
Nutr Hosp. Effect of the “protein diet” and bone tissue. Pubmed: 24483972
Clinical Cancer Research Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivo Aacrjournals: Source
Nutricion Hospitalaria Effect of the “protein diet” and bone tissue Aulamedica: Source
Academic Journal Effect of whole body vibration on healthy rat plasma parameters Ebscohost: Source
J Proteome Res. Biomarkers identified by urinary metabonomics for noninvasive diagnosis of nutritional rickets Pubmed:25051233
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer PubMed: 26266370
J Nutr Sci Vitaminol (Tokyo) The Impact of Different Amounts of Calcium Intake on Bone Mass and Arterial Calcification in Ovariectomized Rats PubMed: 26639847
The Korean Society of Pharmacognosy Effects of Oryza sativa L. Aleurone Layer Extract on Bone Mineral Density and Bone-related Markers in the Ovariectomized Rat Article: Hksobf_2015_V46N2_167
J Bone Miner Res Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082
7 Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. pubmed:28218908
PLOS ONE Prostaglandin-E2 Mediated Increase in Calcium and Phosphate Excretion in a Mouse Model ofDistal Nephron Salt Wasting. pubmed:27442254
International Journal of Advanced Research in Biological Sciences Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat 10.22192/ijarbs.2017.04.04.018
American Journal of Physiology-Renal Physiology Chronic nicotine exposure reduces klotho expression and triggers different renal and hemodynamic responses in -haploinsufficient mice Pubmed:29363324
Tropical Animal Health and Production Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolism Pubmed:29931604
International Immunopharmacology CD8+ T lymphocytes enhance the anabolic effect of intermittent parathyroid hormone on cementoblasts Pubmed: 31679847
ENDOCRINOLOGY A New Therapeutic Approach Using a Calcilytic (AXT914) for Post-Surgical Hypoparathyroidism in Female Rats Pubmed: 32852547
Kidney International Calciprotein particles regulate fibroblast growth factor-23 expression in osteoblasts.
PREVENTIVE AND/OR THERAPEUTIC AGENT FOR OSTEOGENESIS IMPERFECTA AND OTHER DISEASES
Oxid Med Cell Longev Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats 34373766
Gene Therapy Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR1A vector 34599290
Cells Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury Pubmed:35456008
African Health Sciences Fibroblast Growth Factor 23 (FGF 23) and intact parathyroid hormone (iPTH) as markers of mineral bone disease among Nigerians with non-diabetic kidney disease
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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CCA866Ra CLIA Kit for Parathyroid Hormone (PTH) Chemiluminescent immunoassay for Antigen Detection.
LMA866Ra Multiplex Assay Kit for Parathyroid Hormone (PTH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA866Ra11 ELISA Kit DIY Materials for Parathyroid Hormone (PTH) Main materials for "Do It (ELISA Kit) Yourself".