Active Cluster Of Differentiation 146 (CD146)

MCAM; MUC18; Melanoma Cell Adhesion Molecule; Cell surface glycoprotein P1H12; Melanoma-associated antigen A32; S-endo 1 endothelial-associated antigen

ACTIVITY TEST

CD146 (cluster of differentiation 146), also known as MUC18 or MCAM, is a putative adhesion molecule that belongs to the immunoglobulin superfamily (IgSF). It is an approximately 113-kDa type1 transmembrane glycoprotein that is expressed at the intercellular junction of endothelial cells. CD146 was originally identified as a tumor marker for melanoma (MCAM) due to its existence only in melanoma but not in the corresponding normal counterpart. However CD146 is not just a CAM for the inter-cellular and cell-matrix adhesion. Recent evidence indicates that CD146 is actively involved in miscellaneous processes, such as development, signaling transduction, cell migration, mesenchymal stem cells differentiation, angiogenesis and immune response. CD146  and PECAM-1 are both members of immunoglobulin superfamily of cell adhesion molecules. PECAM-1 has been identified as an interactor of CD146, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD146 and recombinant rat PECAM-1. Briefly, CD146 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PECAM-1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CD146 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human CD146 and recombinant rat PECAM-1 was shown in Figure 1, the EC50 for this effect is 3.6 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Clinical Rheumatology Evaluation of various endothelial biomarkers in ankylosing spondylitis PubMed: 21556780
Inflammation The Effect of Regular Colchicine Treatment on Biomarkers Related with Vascular Injury in Newly Diagnosed Patients with Familial Mediterranean Fever SpringerLink: m7128503g576w808
Oncotarget Selective targeting of liver cancer with the endothelial marker CD146 Pubmed:25238265
Future Medicine Melanoma cell adhesion molecule as an emerging biomarker with prognostic significance in systolic heart failure pubmed:27355366
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB670Hu01 Active Cluster Of Differentiation 146 (CD146) Cell culture; Activity Assays.
RPB670Hu01 Recombinant Cluster Of Differentiation 146 (CD146) Positive Control; Immunogen; SDS-PAGE; WB.
PAB670Hu01 Polyclonal Antibody to Cluster Of Differentiation 146 (CD146) WB; ICC/IF
FAB670Hu01 Anti-Cluster Of Differentiation 146 (CD146) Polyclonal Antibody FCM
FAB670Hu81 FITC-Linked Anti-Cluster Of Differentiation 146 (CD146) Polyclonal Antibody FCM
RAB670Hu21 Recombinant Antibody to Cluster Of Differentiation 146 (CD146) WB; IF; ICC; IHC; IP; FCM.
MAB670Hu22 Monoclonal Antibody to Cluster Of Differentiation 146 (CD146) WB; IHC; ICC; IP.
SEB670Hu ELISA Kit for Cluster Of Differentiation 146 (CD146) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB670Hu Multiplex Assay Kit for Cluster Of Differentiation 146 (CD146) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.