Packages (Simulation)
Image (II)
-
Human Hela cell line was stained with 5-10µg/ml mouse Anti-human CD146 Recombinant Antibodies (Catalog # RAB670Hu21, red histogram) or Isotype control antibody (Catalog # IS067-Mu02, green histogram), followed by 1µg/ml PE-conjugated Anti-mouse IgG Secondary Antibody (Catalog # SAA544Mu14).
-
Human peripheral blood lymphocytes were stained with 1µg/ml mouse Anti-human CD146 Recombinant Antibody (Catalog RAB670Hu21, green histogram) or Isotype control antibody (Catalog IS067-Mu03, blue histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18)
Quality Guarantee
Certificate
Recombinant Antibody to Cluster Of Differentiation 146 (CD146)
MCAM; MUC18; Melanoma Cell Adhesion Molecule; Cell surface glycoprotein P1H12; Melanoma-associated antigen A32; S-endo 1 endothelial-associated antigen
- Product No.RAB670Hu21
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceRecombinant monoclonal antibody preparation
- HostMouse
- Potency (Clone Number)n/a
- Ig Isotype IgG
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species Moren/a
- ApplicationsWB; IF; ICC; IHC; IP; FCM.
If the antibody is used in flow cytometry, please check FCM antibodies. - Downloadn/a
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 180
US$ 420
US$ 600
US$ 1500
US$ 6000
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against CD146. It has been selected for its ability to recognize CD146 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:10μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
-
Antibody Labeling Customized Service
-
Protein A/G Purification Column
-
Staining Solution for Cells and Tissue
-
Positive Control for Antibody
-
Tissue/Sections Customized Service
-
Phosphorylated Antibody Customized Service
-
Western Blot (WB) Experiment Service
-
Immunohistochemistry (IHC) Experiment Service
-
Immunocytochemistry (ICC) Experiment Service
-
Flow Cytometry (FCM) Experiment Service
-
Immunoprecipitation (IP) Experiment Service
-
Immunofluorescence (IF) Experiment Service
-
Buffer
-
DAB Chromogen Kit
-
SABC Kit
-
Long-arm Biotin Labeling Kit
-
Mouse Antibody Variable Region Gene Amplification Kit
-
Real Time PCR Experimental Service
| Magazine | Citations |
| Clinical Rheumatology | Evaluation of various endothelial biomarkers in ankylosing spondylitis PubMed: 21556780 |
| Inflammation | The Effect of Regular Colchicine Treatment on Biomarkers Related with Vascular Injury in Newly Diagnosed Patients with Familial Mediterranean Fever SpringerLink: m7128503g576w808 |
| Oncotarget | Selective targeting of liver cancer with the endothelial marker CD146 Pubmed:25238265 |
| Future Medicine | Melanoma cell adhesion molecule as an emerging biomarker with prognostic significance in systolic heart failure pubmed:27355366 |