Packages (Simulation)
Image (I)
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IHC Images
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AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human CD146 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
Image (II)
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Western Blot; Sample: Mouse Uterus lysate Primary Ab: 0.5µg/ml Rabbit Anti-Human CD146 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Lane1: Human Hela cell lysate; Lane2: Human HepG2 cell lysate
Primary Ab: 1µg/mL Rabbit Anti-Human MCAM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Lung lysate
Primary Ab: 0.1µg/ml Rabbit Anti-Human CD146 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Quality Guarantee
Instruction Manual
Certificate
Polyclonal Antibody to Cluster Of Differentiation 146 (CD146)
MCAM; MUC18; Melanoma Cell Adhesion Molecule; Cell surface glycoprotein P1H12; Melanoma-associated antigen A32; S-endo 1 endothelial-associated antigen
- Product No.PAB670Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB670Hu01-Recombinant Cluster Of Differentiation 146 (CD146)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/mL
- Organism Species MoreMus musculus (Mouse)
- ApplicationsWB; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 100
US$ 232
US$ 332
US$ 830
US$ 3320
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CD146. It has been selected for its ability to recognize CD146 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Clinical Rheumatology | Evaluation of various endothelial biomarkers in ankylosing spondylitis PubMed: 21556780 |
| Inflammation | The Effect of Regular Colchicine Treatment on Biomarkers Related with Vascular Injury in Newly Diagnosed Patients with Familial Mediterranean Fever SpringerLink: m7128503g576w808 |
| Oncotarget | Selective targeting of liver cancer with the endothelial marker CD146 Pubmed:25238265 |
| Future Medicine | Melanoma cell adhesion molecule as an emerging biomarker with prognostic significance in systolic heart failure pubmed:27355366 |