Recombinant Protein Customized Service

Instruction manual

First Edition (Revised on April, 2016)

Cloud-Clone Crop. provides one-stop services from genes to proteins. Customers also can choose one or two kinds of services according to their specific requirements. The protein expression platform is composed of prokaryotic expression systems (E.coli) and eukaryotic expression systems (yeast, baculovirus-insect cells, mammalian cells). We are always striving to provide the most comprehensive and high-quality service for every customer based on the continuous innovation and improvement.

Baculovirus-insect cells expression system

1. Multiple labels
2. Virus with high titers virus
3. High efficient V series expression vector and rapid purification technology

E.coli expression system

1. Multiple labels
2. Different expression vectors and host bacteria
3. High efficient C series expression vector and rapid purification technology

Yeast expression system

1. Multiple labals
2. Rapid screening technique on multicopy positive strains
3. High efficient Y series expression vector and rapid purification technology

Mammalian cells expression System

1. Multiple labels
2. High efficient infection technology on mammalian cells 
3. High efficient Y series expression vector and rapid purification technology

Protein purification

1. Different purification methods
2. The technology of rapid removal of endotoxin
3. The technology of tag removal
4. The technology of one-step efficient purification

Refolding of inclusion body

The technology of EASYFOLD

Conventional processes of custom protein

Gene clone or gene synthesis→
Construction of subcloning plasmid→
Construction of expression plasmid→
Optimization of expression conditions→
Protein expression→
Protein purification→
Post processing: endotoxins removal, fusion tag removal, activity tests etc.