Recombinant Neutrophil Elastase (NE)
ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase
- Product No.RPA181Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourceProkaryotic expression
- HostE.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationCytoplasm, Extracellular matrix
- Predicted Molecular Mass27.4kDa
- Accurate Molecular Mass30kDa(Analysis of differences refer to the manual)
- Residues & TagsIle29~Arg247 with N-terminal His Tag
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point10.0
-
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein. - DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 259
For more details, please contact local distributors! US$ 648 US$ 1296 US$ 3888 US$ 9720
SEQUENCE
![](/upload/product/pictures/RPA181Mu01-201903191622256743.jpg)
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Endotoxin Removal Kit
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BCA Protein Quantification Kit
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Protein Labeling Customized Service
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Molecular Mass Marker for Protein
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Recombinant Protein Customized Service
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Buffer
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Endotoxin Removal Kit II
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Real Time PCR Experimental Service
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Magazine | Citations |
Mediators of Inflammation | Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation Hindawi: 612848 |
Journal of Photochemistry and Photobiology B: Biology | A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins Pubmed:25732262 |
International Journal of Molecular Medicine | The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats pubmed:27430552 |
European Journal of Trauma and Emergency | Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model. pubmed:28070608 |
Clin Exp Pharmacol Physiol. | Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. pubmed:28251688 |
Journal of Cardiology | Targeted regulation of sympathetic activity in paraventricular nucleus reduces inducible ventricular arrhythmias in rats after myocardial infarction 10.1016:j.jjcc.2018.06.003 |
Neuroscience | Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats Pubmed:29684507 |
Molecular and Cellular Biochemistry | Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes Pubmed:28948423 |
Neuropsychiatric disease and treatment | The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic … Pubmed:29379286 |
Behavioural Brain Research | Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats Pubmed: 30423388 |
Journal of Pineal Research | Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice Pubmed: 30929267 |
Fundamental & clinical pharmacology | Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo Pubmed: 31313350 |
Cell Death & Disease | Erythromycin suppresses neutrophil extracellular traps in smoking-related chronic pulmonary inflammation Pubmed: 31515489 |
Journal of nutritional science and vitaminology | Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively … Pubmed: 31474682 |
Journal of Clinical Immunology | MiR-608 Exerts Anti-inflammatory Effects by Targeting ELANE in Monocytes Pubmed: 31749032 |
Pathology & Oncology Research | Neutrophil Extracellular Traps Associate with Clinical Stages in Breast Cancer Pubmed: 31656990 |
Bioscience Reports | Inhibition of microRNA-146a attenuated heart failure in myocardial infarction rats Pubmed: 31763669 |
Oxidative Medicine and Cellular Longevity | Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation Pubmed: 31871555 |
Cytokine | Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis Pubmed: 31927460 |
Journal of neural transmission (Vienna, Austria : 1996) | Neurotransmitter system aberrations in patients with drug addiction Pubmed: 32804296 |
experimental eye research | Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis Pubmed: 32376471 |
CLINICAL AND EXPERIMENTAL HYPERTENSION | Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats Pubmed: 32349626 |
Br J Pharmacol | Macrophage Extracellular Traps Aggravate Iron Overload©\Related Liver Ischemia/Reperfusion Injury 33959955 |
Evidence-Based Complementary and Alternative Medicine | Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling?¡ 33505502 |
Nat Sci Sleep | Depression of Non-Neuronal Cholinergic System May Play a Role in Co-Occurrence of Subjective Daytime Sleepiness and Hypertension in Patients with … 34934375 |
Neuromodulation: Technology at the Neural Interface | Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome Pubmed:35088760 |
A Novel Role of Tinospora Cordifolia in Amelioration of Cancer-Induced Systemic Deterioration By Taming Neutrophil Infiltration and Hyperactivation |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
APA181Mu61 | Active Neutrophil Elastase (NE) | Cell culture; Activity Assays. |
RPA181Mu01 | Recombinant Neutrophil Elastase (NE) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA181Mu02 | Recombinant Neutrophil Elastase (NE) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA181Mu61 | Eukaryotic Neutrophil Elastase (NE) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA181Mu01 | Polyclonal Antibody to Neutrophil Elastase (NE) | WB; IHC; ICC; IP. |
PAA181Mu02 | Polyclonal Antibody to Neutrophil Elastase (NE) | WB; IHC; ICC; IP. |
SEA181Mu | ELISA Kit for Neutrophil Elastase (NE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA181Mu | Multiplex Assay Kit for Neutrophil Elastase (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |