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ELISA Kit for Neutrophil Elastase (NE)
ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase
- Product No.SEA181Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.062ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 398
For more details, please contact local distributors! US$ 568 US$ 2556 US$ 4828 US$ 39760
Specificity
This assay has high sensitivity and excellent specificity for detection of Neutrophil Elastase (NE).
No significant cross-reactivity or interference between Neutrophil Elastase (NE) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Neutrophil Elastase (NE) and the recovery rates were calculated by comparing the measured value to the expected amount of Neutrophil Elastase (NE) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 81-101 | 92 |
EDTA plasma(n=5) | 89-96 | 92 |
heparin plasma(n=5) | 95-102 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil Elastase (NE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil Elastase (NE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neutrophil Elastase (NE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 82-94% | 93-105% | 79-89% | 81-101% |
EDTA plasma(n=5) | 95-105% | 82-101% | 82-89% | 80-105% |
heparin plasma(n=5) | 92-105% | 80-94% | 93-101% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Cell Death & Disease | Erythromycin suppresses neutrophil extracellular traps in smoking-related chronic pulmonary inflammation Pubmed: 31515489 |
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Pathology & Oncology Research | Neutrophil Extracellular Traps Associate with Clinical Stages in Breast Cancer Pubmed: 31656990 |
Bioscience Reports | Inhibition of microRNA-146a attenuated heart failure in myocardial infarction rats Pubmed: 31763669 |
Oxidative Medicine and Cellular Longevity | Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation Pubmed: 31871555 |
Cytokine | Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis Pubmed: 31927460 |
Journal of neural transmission (Vienna, Austria : 1996) | Neurotransmitter system aberrations in patients with drug addiction Pubmed: 32804296 |
experimental eye research | Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis Pubmed: 32376471 |
CLINICAL AND EXPERIMENTAL HYPERTENSION | Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats Pubmed: 32349626 |
Br J Pharmacol | Macrophage Extracellular Traps Aggravate Iron Overload©\Related Liver Ischemia/Reperfusion Injury 33959955 |
Evidence-Based Complementary and Alternative Medicine | Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling?¡ 33505502 |
Nat Sci Sleep | Depression of Non-Neuronal Cholinergic System May Play a Role in Co-Occurrence of Subjective Daytime Sleepiness and Hypertension in Patients with … 34934375 |
Neuromodulation: Technology at the Neural Interface | Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome Pubmed:35088760 |
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Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
APA181Mu61 | Active Neutrophil Elastase (NE) | Cell culture; Activity Assays. |
RPA181Mu01 | Recombinant Neutrophil Elastase (NE) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA181Mu02 | Recombinant Neutrophil Elastase (NE) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA181Mu61 | Eukaryotic Neutrophil Elastase (NE) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA181Mu01 | Polyclonal Antibody to Neutrophil Elastase (NE) | WB; IHC; ICC; IP. |
PAA181Mu02 | Polyclonal Antibody to Neutrophil Elastase (NE) | WB; IHC; ICC; IP. |
SEA181Mu | ELISA Kit for Neutrophil Elastase (NE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA181Mu | Multiplex Assay Kit for Neutrophil Elastase (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |