Neutrophil Elastase (NE)

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ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase

Neutrophil Elastase (NE)

Neutrophil elastase is a serine protease in the same family as chymotrypsin and has broad substrate specificity. Secreted by neutrophils during inflammation, it destroys bacteria and host tissue. As with other serine proteases it contains a charge relay system composed of the catalytic triad of histidine, aspartate, and serine residues that are dispersed throughout the primary sequence of the polypeptide but that are brought together in the three dimension conformation of the folded protein. The gene encoding neutrophil elastase, ELA2, consists of five exons.  The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains. It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA181Hu01 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
APA181Hu01 Active Neutrophil Elastase (NE) Cell culture; Activity Assays.
RPA181Hu02 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
RPA181Hu03 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
RPA181Hu04 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
APA181Hu03 Active Neutrophil Elastase (NE) Cell culture; Activity Assays.
Antibodies PAA181Hu01 Polyclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
MAA181Hu22 Monoclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
Assay Kits SEA181Hu ELISA Kit for Neutrophil Elastase (NE) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA181Mu01 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
RPA181Mu02 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
EPA181Mu61 Eukaryotic Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
APA181Mu61 Active Neutrophil Elastase (NE) Cell culture; Activity Assays.
Antibodies PAA181Mu01 Polyclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
PAA181Mu02 Polyclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
Assay Kits SEA181Mu ELISA Kit for Neutrophil Elastase (NE) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA181Ra01 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
APA181Ra01 Active Neutrophil Elastase (NE) Cell culture; Activity Assays.
Antibodies PAA181Ra01 Polyclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
MAA181Ra21 Monoclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
Assay Kits SEA181Ra ELISA Kit for Neutrophil Elastase (NE) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Canis familiaris; Canine (Dog)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA181Ca01 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA181Ca01 Polyclonal Antibody to Neutrophil Elastase (NE) WB; IHC
MAA181Ca21 Monoclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
Assay Kits SEA181Ca ELISA Kit for Neutrophil Elastase (NE) Enzyme-linked immunosorbent assay for Antigen Detection.
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  16. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
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  19. "The refined 2.3-A crystal structure of human leukocyte elastase in a complex with a valine chloromethyl ketone inhibitor."FEBS Lett. 234:367-373(1988) [PubMed] [Europe PMC] [Abstract]
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  23. "Mutations in the ELA2 gene encoding neutrophil elastase are present in most patients with sporadic severe congenital neutropenia but only in some patients with the familial form of the disease."Blood 98:2645-2650(2001) [PubMed] [Europe PMC] [Abstract]
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  27. "A novel mutation Ala57Val of the ELA2 gene in a Korean boy with severe congenital neutropenia."Ann. Hematol. 88:593-595(2009) [PubMed] [Europe PMC] [Abstract]
  28. "Homozygous HAX1 mutations in severe congenital neutropenia patients with sporadic disease: a novel mutation in two unrelated British kindreds."Br. J. Haematol. 144:762-770(2009) [PubMed] [Europe PMC] [Abstract]
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  31. "Pegfilgrastim in children with severe congenital neutropenia."Pediatr. Blood Cancer 54:465-467(2010) [PubMed] [Europe PMC] [Abstract]
  32. "Severe congenital neutropenia in a multigenerational family with a novel neutrophil elastase (ELANE) mutation."Ann. Hematol. 90:151-158(2011) [PubMed] [Europe PMC] [Abstract]
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  34. "The spectrum of ELANE mutations and their implications in severe congenital and cyclic neutropenia."Hum. Mutat. 34:905-914(2013) [PubMed] [Europe PMC] [Abstract]