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ELISA Kit DIY Materials for Klotho (KL)

KLa; Klotho Alpha

  • Product No.KSH757Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range3.12-200pg/mL
  • Applicable Sensitivity1.25pg/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1296  US$ 2160  US$ 3888  US$ 7560  US$ 12960 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Klotho (KL). No significant cross-reactivity or interference between Klotho (KL) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Life Sciences High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculature Pubmed: 24012609
EXPERIMENTAL & CLINICAL CARDIOLOGY Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in Mice Com:Source
Int J Clin Exp Pathol.? α-Klotho is an acute phase protein and altered by restraint stress in mice Pubmed:Pmc4203207
Annales de Biologie Clinique Les biomarqueurs des calcifications vasculaires PubMed: 26069069
Mol Med Rep Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with Klotho PubMed: 25873020
Calcif Tissue Int Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3 PubMed: 25899975
中华内分泌代谢杂志 抗衰老蛋白Klotho 阻断高糖培养的大鼠肾小球系膜细胞TLR4/NF-κB p65/NGAL 通路的激活及其作用机制 Cn: Article
Mol Cell Endocrinol The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanism PubMed: 26419931
Arch Med Sci Soluble Klotho protein as a novel serum biomarker in patients with acromegaly pmc:PMC4754356
Am J Nephrol Klotho Deficiency Aggravates Tacrolimus-Induced Renal Injury via the Phosphatidylinositol 3-Kinase-Akt-Forkhead Box Protein O Pathway Pubmed:27174564
Sci Rep. Klotho preservation via histone deacetylase inhibition attenuates chronic kidney disease-associated bone injury in mice. pubmed:28387374
Nephrology Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management? pubmed:28429560
Journal of Integrative Nephrology & Andrology Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patients issn:2394-2916
International Journal of Clinical and Experimental Pathology Protective effect of restructuring klotho protein combined with trimetazidine on myocardial injury in mice ISSN:1936-2625
Journal of veterinary medical science Changes of Klotho protein and Klotho mRNA expression in a hydroxy-L-proline induced hyperoxaluric rat model pubmed:28943597
Cellular Physiology and Biochemistry Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion Injury Pubmed:29550818
American Journal of Translational Research Advanced maternal age impairs spatial learning capacity in young adult mouse offspring Pubmed:29636887
Journal of Diabetes and its Complications Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complications Pubmed:30042059
Medical Science Monitor Klotho Inhibits Proliferation and Migration of Angiotensin II-Induced Vascular Smooth Muscle Cells (VSMCs) by Modulating NF-κB p65, Akt, and Extracellular Signal … Pubmed:30004089
Nephrology Dialysis Transplantation High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney disease Pubmed: 30189026
BMC Nephrology The role of klotho in chronic kidney disease Pubmed: 30348110
Nature Communications Age-related declines in α-Klotho drive progenitor cell mitochondrial dysfunction and impaired muscle regeneration Pubmed: 30451844
Kidney International Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome Pubmed: 30770217
Expert Opinion on Therapeutic Targets The use of LP533401 as a therapeutic option for renal osteodystrophy affects, renal calcium handling, vitamin D metabolism and bone health in uremic rats Pubmed: 30801205
Mol Immunol CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection Pubmed: 33223223
Journal of Clinical Hypertension Effects of salt intervention on serum levels of Klotho influenced by salt sensitivity Pubmed: 33164306
CURRENT EYE RESEARCH Expression of α-Klotho is downregulated and associated with oxidative stress in the lens in streptozotocin-induced diabetic rats Pubmed: 32744464
international immunopharmacology Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic rats Pubmed: 32438076
Purinergic Signalling P2X7 siRNA targeted to the kidneys increases klotho and delays the progression of experimental diabetic nephropathy. Pubmed: 32377919
Oxidative Medicine and Cellular Longevity Ameliorating Effect of Klotho Protein on Rat Heart during I/R Injury Pubmed: 33123314
NEUROSCIENCE Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With Schizophrenia Pubmed: 32612508
Pharmacological Reports ¦ÁKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy 33675964
J Mol Neurosci Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma Cells Pubmed:35112226
Reproductive Biology and Endocrinology Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance
Journal of Ovarian Research Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPH757Mu03 Recombinant Klotho (KL) Positive Control; Immunogen; SDS-PAGE; WB.
RPH757Mu02 Recombinant Klotho (KL) Positive Control; Immunogen; SDS-PAGE; WB.
RPH757Mu01 Recombinant Klotho (KL) Positive Control; Immunogen; SDS-PAGE; WB.
PAH757Mu02 Polyclonal Antibody to Klotho (KL) WB; IHC; ICC; IP.
PAH757Mu01 Polyclonal Antibody to Klotho (KL) WB; IHC; ICC; IP.
LAH757Mu71 Biotin-Linked Polyclonal Antibody to Klotho (KL) WB; IHC; ICC.
LAH757Mu81 FITC-Linked Polyclonal Antibody to Klotho (KL) WB; IHC; ICC; IF.
SEH757Mu ELISA Kit for Klotho (KL) Enzyme-linked immunosorbent assay for Antigen Detection.
WEH757Mu Wide-range ELISA Kit for Klotho (KL) Enzyme-linked immunosorbent assay for Antigen Detection.
SCH757Mu CLIA Kit for Klotho (KL) Chemiluminescent immunoassay for Antigen Detection.
LMH757Mu Multiplex Assay Kit for Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSH757Mu01 ELISA Kit DIY Materials for Klotho (KL) Main materials for "Do It(ELISA Kit) Yourself"