Recombinant Klotho (KL)
KLa; Klotho Alpha
- Product No.RPH757Mu03
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourceProkaryotic expression
- HostE.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationSecreted
- Predicted Molecular Mass112.5kDa
- Accurate Molecular Mass112kDa(Analysis of differences refer to the manual)
- Residues & TagsAla35~Lys982 with N-terminal His Tag
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 80%
- Isoelectric Point7.2
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein. - DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
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For more details, please contact local distributors!
SEQUENCE
![](/upload/product/pictures/RPH757Mu03-202408011723463160.jpg)
USAGE
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Endotoxin Removal Kit
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BCA Protein Quantification Kit
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Protein Labeling Customized Service
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Molecular Mass Marker for Protein
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Recombinant Protein Customized Service
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Buffer
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Endotoxin Removal Kit II
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Real Time PCR Experimental Service
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Magazine | Citations |
Life Sciences | High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculature Pubmed: 24012609 |
EXPERIMENTAL & CLINICAL CARDIOLOGY | Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in Mice Com:Source |
Int J Clin Exp Pathol.? | α-Klotho is an acute phase protein and altered by restraint stress in mice Pubmed:Pmc4203207 |
Annales de Biologie Clinique | Les biomarqueurs des calcifications vasculaires PubMed: 26069069 |
Mol Med Rep | Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with Klotho PubMed: 25873020 |
Calcif Tissue Int | Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3 PubMed: 25899975 |
中华内分泌代谢杂志 | 抗衰老蛋白Klotho 阻断高糖培养的大鼠肾小球系膜细胞TLR4/NF-κB p65/NGAL 通路的激活及其作用机制 Cn: Article |
Mol Cell Endocrinol | The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanism PubMed: 26419931 |
Arch Med Sci | Soluble Klotho protein as a novel serum biomarker in patients with acromegaly pmc:PMC4754356 |
Am J Nephrol | Klotho Deficiency Aggravates Tacrolimus-Induced Renal Injury via the Phosphatidylinositol 3-Kinase-Akt-Forkhead Box Protein O Pathway Pubmed:27174564 |
Sci Rep. | Klotho preservation via histone deacetylase inhibition attenuates chronic kidney disease-associated bone injury in mice. pubmed:28387374 |
Nephrology | Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management? pubmed:28429560 |
Journal of Integrative Nephrology & Andrology | Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patients issn:2394-2916 |
International Journal of Clinical and Experimental Pathology | Protective effect of restructuring klotho protein combined with trimetazidine on myocardial injury in mice ISSN:1936-2625 |
Journal of veterinary medical science | Changes of Klotho protein and Klotho mRNA expression in a hydroxy-L-proline induced hyperoxaluric rat model pubmed:28943597 |
Cellular Physiology and Biochemistry | Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion Injury Pubmed:29550818 |
American Journal of Translational Research | Advanced maternal age impairs spatial learning capacity in young adult mouse offspring Pubmed:29636887 |
Journal of Diabetes and its Complications | Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complications Pubmed:30042059 |
Medical Science Monitor | Klotho Inhibits Proliferation and Migration of Angiotensin II-Induced Vascular Smooth Muscle Cells (VSMCs) by Modulating NF-κB p65, Akt, and Extracellular Signal … Pubmed:30004089 |
Nephrology Dialysis Transplantation | High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney disease Pubmed: 30189026 |
BMC Nephrology | The role of klotho in chronic kidney disease Pubmed: 30348110 |
Nature Communications | Age-related declines in α-Klotho drive progenitor cell mitochondrial dysfunction and impaired muscle regeneration Pubmed: 30451844 |
Kidney International | Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome Pubmed: 30770217 |
Expert Opinion on Therapeutic Targets | The use of LP533401 as a therapeutic option for renal osteodystrophy affects, renal calcium handling, vitamin D metabolism and bone health in uremic rats Pubmed: 30801205 |
Mol Immunol | CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection Pubmed: 33223223 |
Journal of Clinical Hypertension | Effects of salt intervention on serum levels of Klotho influenced by salt sensitivity Pubmed: 33164306 |
CURRENT EYE RESEARCH | Expression of α-Klotho is downregulated and associated with oxidative stress in the lens in streptozotocin-induced diabetic rats Pubmed: 32744464 |
international immunopharmacology | Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic rats Pubmed: 32438076 |
Purinergic Signalling | P2X7 siRNA targeted to the kidneys increases klotho and delays the progression of experimental diabetic nephropathy. Pubmed: 32377919 |
Oxidative Medicine and Cellular Longevity | Ameliorating Effect of Klotho Protein on Rat Heart during I/R Injury Pubmed: 33123314 |
NEUROSCIENCE | Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With Schizophrenia Pubmed: 32612508 |
Pharmacological Reports | ¦ÁKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy 33675964 |
J Mol Neurosci | Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma Cells Pubmed:35112226 |
Reproductive Biology and Endocrinology | Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance |
Journal of Ovarian Research | Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
RPH757Mu03 | Recombinant Klotho (KL) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPH757Mu02 | Recombinant Klotho (KL) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPH757Mu01 | Recombinant Klotho (KL) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAH757Mu02 | Polyclonal Antibody to Klotho (KL) | WB; IHC; ICC; IP. |
PAH757Mu01 | Polyclonal Antibody to Klotho (KL) | WB; IHC; ICC; IP. |
LAH757Mu71 | Biotin-Linked Polyclonal Antibody to Klotho (KL) | WB; IHC; ICC. |
LAH757Mu81 | FITC-Linked Polyclonal Antibody to Klotho (KL) | WB; IHC; ICC; IF. |
SEH757Mu | ELISA Kit for Klotho (KL) | Enzyme-linked immunosorbent assay for Antigen Detection. |
WEH757Mu | Wide-range ELISA Kit for Klotho (KL) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCH757Mu | CLIA Kit for Klotho (KL) | Chemiluminescent immunoassay for Antigen Detection. |
LMH757Mu | Multiplex Assay Kit for Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSH757Mu01 | ELISA Kit DIY Materials for Klotho (KL) | Main materials for "Do It(ELISA Kit) Yourself" |