ELISA Kit DIY Materials for Regenerating Islet Derived Protein 3 Gamma (REG3g)

REG-III; PAP1B; PAPIB; UNQ429; Pancreatitis-associated protein 1B; Regenerating islet-derived protein III-gamma

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Regenerating Islet Derived Protein 3 Gamma (REG3g). No significant cross-reactivity or interference between Regenerating Islet Derived Protein 3 Gamma (REG3g) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
16 Innate Stat3-mediated induction of the antimicrobial protein Reg3γ is required for host defense against MRSA pneumonia PubMed: PMC3600913
Biology of Blood and Marrow Transplatation Reciprocal Expression of Enteric Antimicrobial Proteins in Intestinal Graft-Versus-Host Disease Pubmed: 23927965
Nature Comunication Hyperglycaemia inhibits REG3A expression to exacerbate TLR3-mediated skin inflammation in diabetes. pubmed:27830702
Gastroenterology Enteric Delivery of Regenerating Family Member 3 alpha Alters the Intestinal Microbiota and Controls Inflammation in Mice With Colitis Pubmed:29133078
Kidney International Interleukin-6/Stat3 signaling has an essential role in the host antimicrobial response to urinary tract infection Pubmed:29475562
Journal of Immunology Research Role of TLR5 in the Translocation and Dissemination of Commensal Bacteria in the Intestine after Traumatic Hemorrhagic Shock 34840990
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KSE676Hu01 ELISA Kit DIY Materials for Regenerating Islet Derived Protein 3 Gamma (REG3g) Main materials for "Do It (ELISA Kit) Yourself".