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ELISA Kit DIY Materials for Ki-67 Protein (Ki-67)

MKI67; KIA; Ki67P; Antigen Identified By Monoclonal Antibody Ki-67

  • Product No.KSC047Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.112ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1260  US$ 2100  US$ 3780  US$ 7350  US$ 12600 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Ki-67 Protein (Ki67P). No significant cross-reactivity or interference between Ki-67 Protein (Ki67P) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
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BMC Research Notes Expressions and clinical significances of c-MET, p-MET and E2f-2 in human gastric carcinoma Pubmed:24393368
Chemistry & Biodiversity Chemical composition of aqueous‐ethanol extract of leaves and its effect on representation of caspase 8, caspase 3 and the proliferation marker Ki67 in intrinsic … Pubmed:29874411
Predictive value of the proliferation marker Ki-67 in patients with acute leukemia undergoing hematopoietic stem cell transplantation
Carbohydrate Polymers A novel exopolysaccharide from marine bacterium Pantoea sp. YU16-S3 accelerates cutaneous wound healing through Wnt/β-catenin pathway Pubmed: 32299547
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KSC047Hu01 ELISA Kit DIY Materials for Ki-67 Protein (Ki-67) Main materials for "Do It (ELISA Kit) Yourself".