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Human Hela cell line was fixed with 70% Ethyl Alcohol (10 min),permeabilised with 0.2% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human Ki-67 Monoclonal Antibody (Catalog FAC047Hu02, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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Anti-Ki-67 Protein (Ki-67) Monoclonal Antibody
MKI67; KIA; Ki67P; Antigen Identified By Monoclonal Antibody Ki-67
- Product No.FAC047Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Potency (Clone Number)C2
- Ig Isotype IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Excitation Laser
- Vol. per test
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Organism Species Moren/a
- ApplicationsFlow cytometry.
- Downloadn/a
- UOM 25test50test 100test 200test 500test
- FOB
US$ 132
US$ 220
US$ 352
US$ 440
US$ 770
For more details, please contact local distributors!
USAGE
Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:10μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Isotype Control
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Real Time PCR Experimental Service
| Magazine | Citations |
| BMC Research Notes | Expressions and clinical significances of c-MET, p-MET and E2f-1 in human gastric carcinoma Pubmed: 24393368 |
| BMC Research Notes | Expressions and clinical significances of c-MET, p-MET and E2f-2 in human gastric carcinoma Pubmed:24393368 |
| Chemistry & Biodiversity | Chemical composition of aqueous‐ethanol extract of leaves and its effect on representation of caspase 8, caspase 3 and the proliferation marker Ki67 in intrinsic … Pubmed:29874411 |
| Predictive value of the proliferation marker Ki-67 in patients with acute leukemia undergoing hematopoietic stem cell transplantation | |
| Carbohydrate Polymers | A novel exopolysaccharide from marine bacterium Pantoea sp. YU16-S3 accelerates cutaneous wound healing through Wnt/β-catenin pathway Pubmed: 32299547 |