ELISA Kit DIY Materials for Arginine (Arg)
(S)-2-Amino-5-Guanidinopentanoic Acid
- Product No.KSB938Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range1.23-100ug/mL
- Applicable Sensitivity0.51ug/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1454
For more details, please contact local distributors! US$ 2424 US$ 4363 US$ 8484 US$ 14544
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Arginine (Arg). No significant cross-reactivity or interference between Arginine (Arg)and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Magazine | Citations |
The Journal of Physiology | Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute toendothelial dysfunction related to insulin resistance in rats and morbidly obese humans. pubmed:26840628 |
Cell Death & Disease | α-ketoglutarate dehydrogenase inhibition counteracts breast cancer-associated lung metastasis Pubmed:29988033 |
Nutrition, Metabolism and Cardiovascular Diseases | Arginine bioavailability and endothelin-1 system in the regulation of vascular function of umbilical vein endothelial cells from intrauterine growth restricted newborns Doi: 10.1016/j.numecd.2018.09.002 |
Nitric Oxide | Effects of dietary nitrate supplementation on microvascular physiology at 4559 m altitude–A randomised controlled trial (Xtreme Alps) Pubmed: 31604146 |
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KSB938Ge11 | ELISA Kit DIY Materials for Arginine (Arg) | Main materials for "Do It (ELISA Kit) Yourself". |