ELISA Kit DIY Materials for Arginine (Arg)

(S)-2-Amino-5-Guanidinopentanoic Acid

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Arginine (Arg). No significant cross-reactivity or interference between Arginine (Arg)and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
The Journal of Physiology Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute toendothelial dysfunction related to insulin resistance in rats and morbidly obese humans. pubmed:26840628
Cell Death & Disease α-ketoglutarate dehydrogenase inhibition counteracts breast cancer-associated lung metastasis Pubmed:29988033
Nutrition, Metabolism and Cardiovascular Diseases Arginine bioavailability and endothelin-1 system in the regulation of vascular function of umbilical vein endothelial cells from intrauterine growth restricted newborns Doi: 10.1016/j.numecd.2018.09.002
Nitric Oxide Effects of dietary nitrate supplementation on microvascular physiology at 4559 m altitude–A randomised controlled trial (Xtreme Alps) Pubmed: 31604146
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