≡

Multiplex Assay Kit for Arginine (Arg) ,etc. by FLIA (Flow Luminescence Immunoassay)

(S)-2-Amino-5-Guanidinopentanoic Acid

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Arginine (Arg) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Arginine (Arg) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Arginine (Arg) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Arginine (Arg) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-98 89
EDTA plasma(n=5) 90-97 93
heparin plasma(n=5) 82-104 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginine (Arg) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginine (Arg) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Arginine (Arg) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-103% 84-93% 98-105% 80-103%
EDTA plasma(n=5) 89-101% 95-103% 83-99% 97-105%
heparin plasma(n=5) 93-102% 87-103% 95-103% 80-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:Arg) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
The Journal of Physiology Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute toendothelial dysfunction related to insulin resistance in rats and morbidly obese humans. pubmed:26840628
Cell Death & Disease α-ketoglutarate dehydrogenase inhibition counteracts breast cancer-associated lung metastasis Pubmed:29988033
Nutrition, Metabolism and Cardiovascular Diseases Arginine bioavailability and endothelin-1 system in the regulation of vascular function of umbilical vein endothelial cells from intrauterine growth restricted newborns Doi: 10.1016/j.numecd.2018.09.002
Nitric Oxide Effects of dietary nitrate supplementation on microvascular physiology at 4559 m altitude–A randomised controlled trial (Xtreme Alps) Pubmed: 31604146
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPB938Ge11 BSA Conjugated Arginine (Arg) Immunogen; SDS-PAGE; WB.
CPB938Ge21 OVA Conjugated Arginine (Arg) Immunogen; SDS-PAGE; WB.
PAB938Ge01 Polyclonal Antibody to Arginine (Arg) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEB938Ge ELISA Kit for Arginine (Arg) Enzyme-linked immunosorbent assay for Antigen Detection.
CCB938Ge CLIA Kit for Arginine (Arg) Chemiluminescent immunoassay for Antigen Detection.
LMB938Ge Multiplex Assay Kit for Arginine (Arg) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB938Ge11 ELISA Kit DIY Materials for Arginine (Arg) Main materials for "Do It (ELISA Kit) Yourself".