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ELISA Kit DIY Materials for Hedgehog Homolog, Sonic (SHH)

HHG1; HLP3; HPE3; SMMCI; SHHN; SHH-N

  • Product No.KSB831Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.59ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1300  US$ 2166  US$ 3899  US$ 7581  US$ 12996 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Hedgehog Homolog, Sonic (SHH). No significant cross-reactivity or interference between Hedgehog Homolog, Sonic (SHH) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
United States Patent Application Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html
Annals of the Rheumatic Diseases Elevated serum levels of sonic hedgehog are associated with fibrotic and vascular manifestations in systemic sclerosis pubmed:28495672
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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KSB831Ra01 ELISA Kit DIY Materials for Hedgehog Homolog, Sonic (SHH) Main materials for "Do It (ELISA Kit) Yourself".