Packages (Simulation)

Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)

Image (I)

DAB staining on IHC-P; Samples: Rat Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Rat SHH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Cerebrum Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Rat SHH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Rat SHH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

WB Image

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Instruction Manual

Polyclonal-Antibody-to-Hedgehog-Homolog--Sonic--SHH--PAB831Ra01.pdf

Certificate

Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)

HHG1; HLP3; HPE3; SMMCI; SHHN; SHH-N

Product No.PAB831Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen n/a
Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreHomo sapiens (Human), Mus musculus (Mouse)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 109 253 US$ 253 US$ 362 US$ 905 US$ 3620
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against SHH. It has been selected for its ability to recognize SHH in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
United States Patent Application	Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html
Annals of the Rheumatic Diseases	Elevated serum levels of sonic hedgehog are associated with fibrotic and vascular manifestations in systemic sclerosis pubmed:28495672

Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPB831Ra01	Recombinant Hedgehog Homolog, Sonic (SHH)	Positive Control; Immunogen; SDS-PAGE; WB.
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PAB831Ra01	Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)	WB; IHC; ICC; IP.
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SEB831Ra	ELISA Kit for Hedgehog Homolog, Sonic (SHH)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB831Ra	Multiplex Assay Kit for Hedgehog Homolog, Sonic (SHH) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
KSB831Ra01	ELISA Kit DIY Materials for Hedgehog Homolog, Sonic (SHH)	Main materials for "Do It (ELISA Kit) Yourself".