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ELISA Kit DIY Materials for Myostatin (MSTN)

GDF8; Growth Differentiation Factor 8

  • Product No.KSB653Ra11
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range1.11-90ng/mL
  • Applicable Sensitivity0.44ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1300  US$ 2166  US$ 3899  US$ 7581  US$ 12996 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Myostatin (MSTN). No significant cross-reactivity or interference between Myostatin (MSTN) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Clinical Medicine and Diagnostics Anti-Myostatin Reduces Bone Mineral Loss in Ovariectomized Rats Sapub:Source
Nutr Metab (Lond) Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in mice PubMed: 26539241
International Journal of Cancer Tumor inoculation site affects the development of cancer cachexia and muscle wasting PubMed: 26016447
Connect Tissue Res Induction of transient tenogenic phenotype of high density cultured human dermal fibroblasts PubMed: 25748814
PLoS One Higher Plasma Myostatin Levels in Cor Pulmonale Secondary to Chronic Obstructive Pulmonary Disease Pubmed:26998756
Calcif Tissue Int. The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats Pubmed:27272029
Archives of medical research Irisin and Myostatin Levels in Patients with Graves' Disease. pubmed:27986127
Calcified Tissue International Effect of Strontium Ranelate on the Muscle and Vertebrae of Ovariectomized Rats pubmed:29242963
Scientific Reports Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis. pubmed:28740214
Journal of Animal Science Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigs Pubmed: 30203098
European Journal of Internal Medicine Predictive value of serum myostatin for the severity and clinical outcome of heart failure Pubmed: 31056368
J Cell Physiol Resistance training‐induced muscle hypertrophy is mediated by TGF‐β1‐Smad signaling pathway in male Wistar rats Pubmed: 31960436
NEPHROLOGY DIALYSIS TRANSPLANTATION Myostatin and muscle atrophy during chronic kidney disease Pubmed: 32974666
Oxid Med Cell Longev Gender-Specific Behaviour in Obesity Stages I-II: Imbalance of Aminothiol Status and Adipomyokine Profile in Subjects with Different Insulin Resistance … 34868459
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB653Ra01 Recombinant Myostatin (MSTN) Positive Control; Immunogen; SDS-PAGE; WB.
APB653Ra01 Active Myostatin (MSTN) Cell culture; Activity Assays.
CPB653Ra21 OVA Conjugated Myostatin (MSTN) Immunogen; SDS-PAGE; WB.
PAB653Ra08 Polyclonal Antibody to Myostatin (MSTN) WB; IHC; ICC; IP.
PAB653Ra01 Polyclonal Antibody to Myostatin (MSTN) WB,IHC
LAB653Ra71 Biotin-Linked Polyclonal Antibody to Myostatin (MSTN) WB; IHC; ICC.
MAB653Ra21 Monoclonal Antibody to Myostatin (MSTN) WB; IHC; ICC; IP.
SEB653Ra ELISA Kit for Myostatin (MSTN) Enzyme-linked immunosorbent assay for Antigen Detection.
CEB653Ra ELISA Kit for Myostatin (MSTN) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB653Ra CLIA Kit for Myostatin (MSTN) Chemiluminescent immunoassay for Antigen Detection.
LMB653Ra Multiplex Assay Kit for Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB653Ra11 ELISA Kit DIY Materials for Myostatin (MSTN) Main materials for "Do It (ELISA Kit) Yourself".