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Multiplex Assay Kit for Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay)
GDF8; Growth Differentiation Factor 8
(Note: Up to 8-plex in one testing reaction)
- Product No.LMB653Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range0.09-90ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.03 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 450
US$ 468
US$ 494
US$ 528
US$ 563
US$ 615
US$ 693
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 98-105 | 101 |
EDTA plasma(n=5) | 98-105 | 102 |
heparin plasma(n=5) | 85-99 | 93 |
sodium citrate plasma(n=5) | 89-103 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 84-101% | 80-98% | 87-104% | 87-101% |
EDTA plasma(n=5) | 86-101% | 84-94% | 86-95% | 91-99% |
heparin plasma(n=5) | 84-91% | 94-102% | 83-104% | 86-101% |
sodium citrate plasma(n=5) | 81-101% | 94-103% | 92-101% | 91-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:MSTN) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Clinical Medicine and Diagnostics | Anti-Myostatin Reduces Bone Mineral Loss in Ovariectomized Rats Sapub:Source |
Nutr Metab (Lond) | Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in mice PubMed: 26539241 |
International Journal of Cancer | Tumor inoculation site affects the development of cancer cachexia and muscle wasting PubMed: 26016447 |
Connect Tissue Res | Induction of transient tenogenic phenotype of high density cultured human dermal fibroblasts PubMed: 25748814 |
PLoS One | Higher Plasma Myostatin Levels in Cor Pulmonale Secondary to Chronic Obstructive Pulmonary Disease Pubmed:26998756 |
Calcif Tissue Int. | The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats Pubmed:27272029 |
Archives of medical research | Irisin and Myostatin Levels in Patients with Graves' Disease. pubmed:27986127 |
Calcified Tissue International | Effect of Strontium Ranelate on the Muscle and Vertebrae of Ovariectomized Rats pubmed:29242963 |
Scientific Reports | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis. pubmed:28740214 |
Journal of Animal Science | Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigs Pubmed: 30203098 |
European Journal of Internal Medicine | Predictive value of serum myostatin for the severity and clinical outcome of heart failure Pubmed: 31056368 |
J Cell Physiol | Resistance training‐induced muscle hypertrophy is mediated by TGF‐β1‐Smad signaling pathway in male Wistar rats Pubmed: 31960436 |
NEPHROLOGY DIALYSIS TRANSPLANTATION | Myostatin and muscle atrophy during chronic kidney disease Pubmed: 32974666 |
Oxid Med Cell Longev | Gender-Specific Behaviour in Obesity Stages I-II: Imbalance of Aminothiol Status and Adipomyokine Profile in Subjects with Different Insulin Resistance … 34868459 |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
RPB653Ra01 | Recombinant Myostatin (MSTN) | Positive Control; Immunogen; SDS-PAGE; WB. |
APB653Ra01 | Active Myostatin (MSTN) | Cell culture; Activity Assays. |
CPB653Ra21 | OVA Conjugated Myostatin (MSTN) | Immunogen; SDS-PAGE; WB. |
PAB653Ra08 | Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
PAB653Ra01 | Polyclonal Antibody to Myostatin (MSTN) | WB,IHC |
LAB653Ra71 | Biotin-Linked Polyclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC. |
MAB653Ra21 | Monoclonal Antibody to Myostatin (MSTN) | WB; IHC; ICC; IP. |
SEB653Ra | ELISA Kit for Myostatin (MSTN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEB653Ra | Mini Samples ELISA Kit for Myostatin (MSTN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEB653Ra | ELISA Kit for Myostatin (MSTN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB653Ra | CLIA Kit for Myostatin (MSTN) | Chemiluminescent immunoassay for Antigen Detection. |
LMB653Ra | Multiplex Assay Kit for Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSB653Ra11 | ELISA Kit DIY Materials for Myostatin (MSTN) | Main materials for "Do It (ELISA Kit) Yourself". |