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ELISA Kit DIY Materials for Beta Actin (ACTB)

Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed

  • Product No.KSB340Mi01
  • Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampletissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.57ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • DownloadInstruction Manual
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Actin Beta (ACTb).No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Cell Metabolism Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes
Biological & Pharmaceutical Bulletin Neuroinflammation, Oxidative Stress, and Neurogenesis in a Mouse Model of Chronic Fatigue Syndrome, and the Treatment with Kampo Medicine Pubmed: 31902915
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J Ethnopharmacol Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn. Pubmed:35398498
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Catalog No. Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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KSB340Mi01 ELISA Kit DIY Materials for Beta Actin (ACTB) Main materials for "Do It (ELISA Kit) Yourself".
PGB340Mi01 Primer Pair for Beta Actin (ACTB) PCR