Packages (Simulation)
Reagent Preparation
Image (I)
-
Results demonstration
Image (II)
-
Typical Standard Curve
Quality Guarantee
Instruction Manual
Certificate
Multiplex Assay Kit for Beta Actin (ACTB) ,etc. by CBA (Cytometric Bead Array)
Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
- Product No.CMB340Mi
- Organism Species Same name, Different species.
- Sample TypeTissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length4h
- Detection Range0.0977-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.0326ng/mL.
- DownloadInstruction Manual
- UOM 48T96T
- FOB
US$ 575
US$ 821
For more details, please contact local distributors!
Specificity
This assay has high sensitivity for detection of Beta Actin (ACTB) ,etc. by CBA (Cytometric Bead Array).
100% cross-reactivity of Beta Actin (ACTB) ,etc. by CBA (Cytometric Bead Array) was observed among .
Recovery
Matrices listed below were spiked with certain level of recombinant Beta Actin (ACTB) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Beta Actin (ACTB) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 84-105 | 98 |
| EDTA plasma(n=5) | 95-103 | 98 |
| heparin plasma(n=5) | 80-91 | 85 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta Actin (ACTB) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta Actin (ACTB) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta Actin (ACTB) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-95% | 78-92% | 78-101% | 94-101% |
| EDTA plasma(n=5) | 91-98% | 85-104% | 92-104% | 78-104% |
| heparin plasma(n=5) | 78-92% | 89-97% | 82-99% | 94-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
GIVEAWAYS
INCREMENT SERVICES
| Magazine | Citations |
| International Journal of Molecular Sciences | Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles NCBI:PMC3856104 |
| European Journal of BioMedical Research | Expression of chloride channels in mouse skin incisional wounds 96 |
| Cell Metabolism | Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes |
| Biological & Pharmaceutical Bulletin | Neuroinflammation, Oxidative Stress, and Neurogenesis in a Mouse Model of Chronic Fatigue Syndrome, and the Treatment with Kampo Medicine Pubmed: 31902915 |
| Biological research | Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor … Pubmed: 32046779 |
| JOURNAL OF INVESTIGATIVE MEDICINE | Effect of ischemic postconditioning on cell apoptosis and expression of relevant genes in non-culprit coronary arteries Pubmed: 32784207 |
| bioscience biotechnology research asia | Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring |
| Genes | Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells 33261077 |
| Nat Commun | POLRMT mutations impair mitochondrial transcription causing neurological disease 33602924 |
| Life Sci | Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexus Pubmed:34990649 |
| J Ethnopharmacol | Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn. Pubmed:35398498 |
| Multimedia Tools and Applications | Investigation of single beam ultrasound sensitivity as a monitoring tool for local hyperthermia treatment in breast cancer |