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ELISA Kit DIY Materials for Toll Like Receptor 4 (TLR4)
CD284; TOLL; HToll
- Product No.KSA753Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampletissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.118ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1134
US$ 1890
US$ 3402
US$ 6615
US$ 11340
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Toll Like Receptor 4 (TLR4). No significant cross-reactivity or interference between Toll Like Receptor 4 (TLR4) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Chinese Medical Journal | Expression of soluble Toll-like receptors in pleural effusions CMJ: 2010823483999506297 |
| Journal of Maternal-Fetal and Neonatal Medicine | Amniotic fluid soluble Toll-like receptor 4 in pregnancies complicated by preterm prelabor rupture of the membranes Informa: 14767058.2011.626821 |
| PLoS One | Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation PubMed: PMC3639905 |
| Clin Res Hepatol Gastroenterol. | The association between precancerous gastric lesions and serum pepsinogens, serum gastrin, vascular endothelial growth factor, serum interleukin-1 Beta, serum toll-like receptor-4 levels and Helicobacter pylori Cag A status Pubmed: 23137754 |
| Journal of Clinical Microbiology | Toll-Like Receptor 2 in Serum: a Potential Diagnostic Marker of Prosthetic Joint Infection? Pubmed: 24478497 |
| Surgical Endoscopy | CO2 pneumoperitoneum increases secretory IgA levels in the gut compared with laparotomy in an experimental animal model. Pubmed: 24442682 |
| Experimental and therapeutic medicine | Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietin Pubmed: 24223644 |
| Free Radical Biology and Medicine | Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis Pubmed:24992835 |
| Respiratory Virology and Immunogenicity Advances in Experimental Medicine and Biology | Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination Pubmed:25248345 |
| Molecular and Cellular Biochemistry | Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharide Pubmed:25318610 |
| Journal of Ginseng Research | Effects of Korean Red Ginseng (Panax ginseng), urushiol (Rhus vernicifera Stokes), and probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus acidophilus R0052) on the gut–liver axis of alcoholic liver disease Pubmed:25378990 |
| BMC Immunology | The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases Pubmed:25406630 |
| Gastroenterology Research and Practice | Procoagulatory State in Inflammatory Bowel Diseases Is Promoted by Impaired Intestinal Barrier Function Pubmed:25767508 |
| Eur J Gastroenterol Hepatol | Efficacy of rifaximin on circulating endotoxins and cytokines in patients with nonalcoholic fatty liver disease PubMed: 26043290 |
| Pathol Biol (Paris) | Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice PubMed: 25834936 |
| Journal of Food Science | Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and Cytokine Pubmed:27061742 |
| Plos One | Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage. Pubmed:27223696 |
| Open Forum Infect Dis | The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-Patients Pubmed:26835476 |
| Animal Feed Science and Technology | Impact of Saccharomyces cerevisiae fermentation product and subacute ruminal acidosis on production, inflammation, and fermentation in the rumen and hindCavia (Guinea pig )t of dairy cows science:S0377840115300493 |
| european journal of pharmacology | Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms. pubmed:27915041 |
| european journal of pharmacology | S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase. pubmed:27887948 |
| Psychopharmacology (Berl) | Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms. pubmed:28303372 |
| ЭНИ Забайкальский медицинский вестник | НОСИТЕЛЬСТВО ПОЛИМОРФИЗМА Thr399Ile и Asp299Gly ГЕНА TLR4 И УРОВЕНЬ TLR4 В ОПУХОЛЕВОЙ ТКАНИ У БОЛЬНЫХ ПРОЛИФЕРАТИВНЫМИ ЗАБОЛЕВАНИЯМИ МОЛОЧНОЙ ЖЕЛЕЗЫ 8.pdf |
| biomedicine & pharmacotherapy | Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. pubmed:28199883 |
| Journal For Neurovirology | Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions. pubmed:27882497 |
| Acta Orthopaedica | Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic joints pubmed:28093922 |
| Environmental Toxicology and Pharmacology | Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study pubmed:28192751 |
| Inflammation | Toll-Like Receptor 4 Inhibition Improves Oxidative Stress and Mitochondrial Health in Isoproterenol-Induced Cardiac Hypertrophy in Rats pubmed:28690610 |
| Comparative Immunology, Microbiology and Infectious Diseases | Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts DOI: 10.1016/j.cimid.2017.06.002 |
| Comparative Immunology, Microbiology and Infectious Diseases | Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts 10.1016/j.cimid.2017.06.002 |
| Microbial Pathogenesis | Protective effects of sinomenine against LPS-induced inflammation in piglets. pubmed:28757275 |
| Cytokine | Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects pubmed:29102164 |
| Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms pubmed:28061403 |
| Frontiers in Immunology | The soluble Form of Toll-like receptor 2 is elevated in serum of Multiple sclerosis Patients: a novel Potential Disease Biomarker Pubmed:29593720 |
| Experimental Gerontology | Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia Pubmed:30071285 |
| Neurotoxicity Research | Protective Effects of Fibroblast Growth Factor 21 Against Amyloid-Beta-Induced Toxicity in SH-SY5Y Cells Pubmed:29869772 |
| European Journal of Pharmacology | Rutin, a quercetin glycoside, alleviates acute endotoxemic kidney injury in C57BL/6 mice via suppression of inflammation and up-regulation of antioxidants and SIRT1 Pubmed:29920283 |
| Environmental Toxicology and Pharmacology | Crocin reverses unilateral renal ischemia reperfusion injury-induced augmentation of oxidative stress and toll like receptor-4 activity Pubmed:29625388 |
| Physiology & Behavior | Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll … Pubmed:29410018 |
| International Journal of Gynecology & Obstetrics | Increased serum levels of high mobility group protein B1 and calprotectin in pre‐eclampsia Pubmed:29569400 |
| Journal of Maternal-Fetal & Neonatal Medicine | Increased TLR4 and TREM-1 expression on monocytes and neutrophils in preterm birth: further evidence of a proinflammatory state Pubmed:29534643 |
| ТLR4-РІВНІ У ХВОРИХ НА ПОДАГРУ: ЗВ'ЯЗОК З АКТИВНІСТЮ ЗАХВОРЮВАННЯ : | |
| Journal of Medical Virology | Combined effect of anti‐high‐mobility group box‐1 monoclonal antibody and peramivir against influenza A virus‐induced pneumonia in mice Pubmed: 30281823 |
| Metabolic Brain Disease | Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanisms Pubmed: 30456649 |
| Experimental and Therapeutic Medicine | The HMGB1‑RAGE/TLR‑TNF‑α signaling pathway may contribute to kidney injury induced by hypoxia |
| Immunopharmacology and immunotoxicology | Protective effect of sesamin in lipopolysaccharide-induced mouse model of acute kidney injury via attenuation of oxidative stress, inflammation, and apoptosis Pubmed: 30488751 |
| International Immunopharmacology | S-allyl cysteine protects against lipopolysaccharide-induced acute kidney injury in the C57BL/6 mouse strain: Involvement of oxidative stress and inflammation Pubmed: 30665040 |
| Biomedicine & Pharmacotherapy | Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4 |
| International Journal of Obesity | Circulating microbiota-derived metabolites: a “liquid biopsy? Pubmed: 31388096 |
| Scientific Reports | Deregulation of TLR4 signaling pathway characterizes Bicuspid Aortic valve syndrome Pubmed: 31363123 |
| Life Sciences | Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis Pubmed: 31465731 |
| Respiratory physiology & neurobiology | Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosis Pubmed: 31600583 |
| Thrombosis and haemostasis | Particulate Shiga Toxin 2 in Blood is Associated to the Development of Hemolytic Uremic Syndrome in Children Pubmed: 31858520 |
| J Ethnopharmacol | Effects of fermented ginseng on the gut microbiota and immunity of rats with antibiotic-associated diarrhea Pubmed: 33217518 |
| International Journal of Molecular Sciences | Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis Pubmed: 32545403 |
| NEUROPSYCHOBIOLOGY | Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying Mechanisms Pubmed: 32526752 |
| journal of biochemical and molecular toxicology | S‐allyl cysteine, an active ingredient of garlic, attenuates acute liver dysfunction induced by lipopolysaccharide/ d‐galactosamine in mouse: Underlying mechanisms Pubmed: 32453893 |
| microbial pathogenesis | Protective effect of diosgenin on LPS/D-Gal-induced acute liver failure in C57BL/6 mice Pubmed: 32389705 |
| Sciences of Europe | FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE … |
| Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial … | |
| The role of Toll-like receptors-4 in the pathogenesis of development of anemia of inflammation in young children | |
| Clin Oral Investig | Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces 34091792 |
| Eksp Klin Gastroenterol | Tissue transglutaminase is involved in the inflammatory processes of active chronic gastritis 17539352 |
| Iranian Journal of Medical Sciences | Esculetin Alleviates Acute Liver Failure following Lipopolysaccharide/D-Galactosamine in Male C57BL/6 Mice |
| Acta Obstet Gynecol Scand | A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis 34244993 |
| Nutrients | Expression of Jejunal Taste Receptors in Women with Morbid Obesity 34371946 |
| Biomed Pharmacother | Nifuroxazide-loaded cubosomes exhibit an advancement in pulmonary delivery and attenuate bleomycin-induced lung fibrosis by regulating the STAT3 and … Pubmed:35220029 |
| Biomed Pharmacother | Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an … Pubmed:35202914 |
| Frontiers in Pharmacology | Carbocisteine as a Modulator of Nrf2/HO-1 and NFκB Interplay in Rats: New Inspiration for the Revival of an Old Drug for Treating Ulcerative Colitis. Front … Pubmed:35754464 |
| Processes | Lipocalin, Resistin and Gut Microbiota-Derived Propionate Could Be Used to Predict Metabolic Bariatric Surgery Selected Outcomes |
| Biomolecules | Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study |