≡

ELISA Kit DIY Materials for Immunoglobulin E (IgE)

IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region

  • Product No.KSA545Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.69ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1134  US$ 1890  US$ 3402  US$ 6615  US$ 11340 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Immunoglobulin E (IgE).No significant cross-reactivity or interference between Immunoglobulin E (IgE) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support Pack

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Molecular Carcinogenesis IL17A gene polymorphisms, serum IL-17A and IgE levels, and hepatocellular carcinoma risk in patients with chronic hepatitis B virus infection PubMed: 23280722
Human Immunology IL21 and IL21R polymorphisms and their interactive effects on serum IL-21 and IgE levels in patients with chronic hepatitis B virus infection PubMed: 23354321
Scand J Immunol Passive transfer of tumour-derived MDSCs inhibits asthma-related airway inflammation Pubmed: 24313384
AAPS PharmSciTech Topical Lyogel Containing Corticosteroid Decreases IgE Expression and Enhances the Therapeutic Efficacy Against Atopic Eczema PubMed: 25511806
Ann Allergy Asthma Immunol Reduced airway microbiota diversity is associated with elevated allergic respiratory inflammation PubMed: 26123423
Molecular Medicine Reports The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway. pubmed:27484042
Gut Pathog. Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice pubmed:28250847
International Immunopharmacology Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung pubmed:27992791
Asian Pacific Society of Respirology Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice pubmed:28122397
Molecular Medicine Reports Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. pubmed:28586019
Journal of Autoimmunity Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice 10.1016:j.jaut.2018.07.003
Water Research  Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model Pubmed:29655095
Human & Experimental Toxicology Investigation of hypersensitivity potential of diacetyl by determining cytokine profiles Pubmed:29233007
Journal of International Medical Research Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease Pubmed:29950127
Journal Of Cellular Biochemistry Effects of catalpol on bronchial asthma and its relationship with cytokines Pubmed: 30536454
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES  Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model Pubmed: 30781605
Royal Society Open Science Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats
Health and Medicine Antiallergical Effect of New Combined Nazal Aerodisperse System in the Conditions of Experimental Allergic Rhinitis
American Journal of Translational Research Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression Pubmed: 31737193
MOLECULAR IMMUNOLOGY Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection Pubmed: 32302806
bioscience reports Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat Pubmed: 32395767
international journal of antimicrobial agents Synergistic Phage-surfactant Combination Clears IgE-promoted Staphylococcus aureus Aggregation in Vitro and Enhances the Effect in Vivo Pubmed: 32335278
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
ACS Biomaterials Science & Engineering Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction
Biomarkers of Allergic Asthma and their Association with Serum Parameters
Biomed Pharmacother Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice 33706133
Environ Pollut Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure 34090073
Annals of Tropical Medicine & Public Health Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity
Mediators Inflamm Roles Played by the PI3K/Akt/HIF-1α Pathway and IL-17A in the Chinese Subtype of Chronic Sinusitis with Nasal Polyps Pubmed:35075349
Expert Opinion on Therapeutic Targets Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma Pubmed:35549595
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
NPA545Hu01 Native Immunoglobulin E (IgE) Positive Control; Immunogen; SDS-PAGE; WB.
RPA545Hu01 Recombinant Immunoglobulin E (IgE) Positive Control; Immunogen; SDS-PAGE; WB.
PAA545Hu01 Polyclonal Antibody to Immunoglobulin E (IgE) WB; IHC; ICC; IP.
LAA545Hu71 Biotin-Linked Polyclonal Antibody to Immunoglobulin E (IgE) WB; IHC; ICC.
RAA545Hu21 Recombinant Antibody to Immunoglobulin E (IgE) WB; IF; ICC; IHC; IP; FCM.
MAA545Hu22 Monoclonal Antibody to Immunoglobulin E (IgE) WB; IHC; ICC; IP.
SEA545Hu ELISA Kit for Immunoglobulin E (IgE) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA545Hu Multiplex Assay Kit for Immunoglobulin E (IgE) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA545Hu01 ELISA Kit DIY Materials for Immunoglobulin E (IgE) Main materials for "Do It (ELISA Kit) Yourself".