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Active Immunoglobulin E (IgE)

IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region

ACTIVITY TEST

Immunoglobulin E (IgE) is one of the members the 5 classes of immunoglobulins (IgM, IgG, IgD, IgA, IgE).It is produced by plasma cells and accounts for less than 0.01% of the total immunoglobulin.IgE has a unique chemical structure and various physiological functions, such as type I hypersensitivity reactions, parasitic infections, autoimmune processes, and venom protection. Besides,Cluster of Differentiation 79B (CD79B) has been identified as an interactor of IgE, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IgE and recombinant human CD79B.Briefly, IgE was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CD79B-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IgE pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human IgE and recombinant human CD79B was shown in Figure 1, the EC50 for this effect is 0.075ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Molecular Carcinogenesis IL17A gene polymorphisms, serum IL-17A and IgE levels, and hepatocellular carcinoma risk in patients with chronic hepatitis B virus infection PubMed: 23280722
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Ann Allergy Asthma Immunol Reduced airway microbiota diversity is associated with elevated allergic respiratory inflammation PubMed: 26123423
Molecular Medicine Reports The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway. pubmed:27484042
Gut Pathog. Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice pubmed:28250847
International Immunopharmacology Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung pubmed:27992791
Asian Pacific Society of Respirology Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice pubmed:28122397
Molecular Medicine Reports Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. pubmed:28586019
Journal of Autoimmunity Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice 10.1016:j.jaut.2018.07.003
Water Research  Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model Pubmed:29655095
Human & Experimental Toxicology Investigation of hypersensitivity potential of diacetyl by determining cytokine profiles Pubmed:29233007
Journal of International Medical Research Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease Pubmed:29950127
Journal Of Cellular Biochemistry Effects of catalpol on bronchial asthma and its relationship with cytokines Pubmed: 30536454
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES  Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model Pubmed: 30781605
Royal Society Open Science Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats
Health and Medicine Antiallergical Effect of New Combined Nazal Aerodisperse System in the Conditions of Experimental Allergic Rhinitis
American Journal of Translational Research Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression Pubmed: 31737193
MOLECULAR IMMUNOLOGY Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection Pubmed: 32302806
bioscience reports Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat Pubmed: 32395767
international journal of antimicrobial agents Synergistic Phage-surfactant Combination Clears IgE-promoted Staphylococcus aureus Aggregation in Vitro and Enhances the Effect in Vivo Pubmed: 32335278
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
ACS Biomaterials Science & Engineering Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction
Biomarkers of Allergic Asthma and their Association with Serum Parameters
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Expert Opinion on Therapeutic Targets Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma Pubmed:35549595
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