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ELISA Kit DIY Materials for Endostatin (ES)

  • Product No.KSA542Hu11
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range4.94-400ng/mL
  • Applicable Sensitivity1.74ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1129  US$ 1881  US$ 3386  US$ 6584  US$ 11286 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Endostatin (ES). No significant cross-reactivity or interference between Endostatin (ES) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Cancer Immunology Endostatin gene therapy enhances the efficacy of IL-2 in suppressing metastatic renal cell carcinoma in mice SpringerLink: u38777121t6660l5
Journal of Fluoresence Erythrocyte Protoporphyrin Fluorescence as a Biomarker for Monitoring Antiangiogenic Cancer Therapy SpringerLink: 4v306130302257w0
The Journal of Gene Medicine Endostatin- and interleukin-2-expressing retroviral bicistronic vector for gene therapy of metastatic renal cell carcinoma Wiley: source
Biomedicine & Pharmacotherapy Endostatin neoadjuvant gene therapy extends survival in an orthotopic metastatic mouse model of renal cell carcinoma ScienceDirect: S075333221100151X
Science Alert Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats Scialert: Source
The Journal of Immunology Inflammation Induced by MMP-9 Enhances Tumor Regression of Experimental Breast Cancer PubMed: PMC3619527
Prostaglandins & Other Lipid Mediators Promotion of adipogenesis by an EP2 receptor agonist via stimulation of angiogenesis in pulmonary emphysema Pubmed:24911647
American Journal of Physiology - Heart and Circulatory Physiology Endostatin and kidney fibrosis in aging: a case for antagonistic pleiotropy? Source
shock Angiogenesis inhibitor endostatin protects mice with sepsis from multiple organ dysfunction syndrome PubMed: 26125086
Clin Rev Allergy Immunol Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the Literature PubMed: 26142066
Angiologia e Cirurgia Vascular Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkers science:S1646706X16300052
Clinical Rheumatology Impaired angiogenesis as a feature of digital ulcers in systemic sclerosis Pubmed:26920752
Blood biomarker for early blood brain barrier disruption in ischemic stroke patent:US20170199205A1
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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KSA542Hu11 ELISA Kit DIY Materials for Endostatin (ES) Main materials for "Do It (ELISA Kit) Yourself".