ELISA Kit DIY Materials for Dihydrotestosterone (DHT)

5α-DHT; 5α-Dihydrotestosterone; Androstanolone; Stanolone

  • Product No.KSA443Ge11
  • Organism SpeciesPan-species (General) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range30.9-2500pg/mL
  • Applicable Sensitivity13.3pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1433  US$ 2388  US$ 4298  US$ 8358  US$ 14328 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Dihydrotestosterone (DHT). No significant cross-reactivity or interference between Dihydrotestosterone (DHT) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Internation Scholarly Research Network Herbicide Metolachlor Causes Changes in Reproductive Endocrinology of Male Wistar Rats Isrn: 130846
Journal of American Science Metabolic Changes and Hormonal Disturbances in Polycystic Ovarian Syndrome Rats and the Amelioration Effects of Metformin and/or Cinnamon Extraction Jofamericanscience: Source
Journal of Ethnopharmacology Treatment of benign prostatic hyperplasia with Croton membranaceus in an experimental animal model Pubmed:25256687
Toxicol Ind Health Oral administration of low-dose bisphenol A promotes proliferation of ventral prostate and upregulates prostaglandin D2 PubMed: 26088557
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα pubmed:27413362
Neurochemical Research Dihydrotestosterone Treatment Accelerates Autograft Reversal Sciatic Nerve Regeneration in Rats Pubmed:29340845
Journal of Endocrinology Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146
Journal of Cellular Physiology Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression Pubmed: 30671954
PHYTOTHERAPY RESEARCH Plant cell culture extract of Cirsium eriophorum with skin pore refiner activity by modulating sebum production and inflammatory response Pubmed: 32816329
Curr Oncol Plasma Aromatase Activity Index, Gonadotropins and Estrone Are Associated with Frailty Syndrome in Post-Menopausal Women with Breast Cancer Pubmed:35323344
J Steroid Biochem Mol Biol Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells Pubmed:35697131
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
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KSA443Ge11 ELISA Kit DIY Materials for Dihydrotestosterone (DHT) Main materials for "Do It (ELISA Kit) Yourself".