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ELISA Kit DIY Materials for Angiopoietin 2 (ANGPT2)

ANG2; AGPT2

  • Product No.KSA009Bo01
  • Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range12.5-800pg/mL
  • Applicable Sensitivity5.4pg/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1360  US$ 2267  US$ 4081  US$ 7935  US$ 13602 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Angiopoietin 2 (ANGPT2). No significant cross-reactivity or interference between Angiopoietin 2 (ANGPT2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Microvascular Research Mutant hypoxia inducible factor-1α improves angiogenesis and tissue perfusion in ischemic rabbit skeletal muscle PubMed: 20937289
Theriogenology Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes Science: Article
Pediatr Infect Dis J Important of Angiopoietic System in Evaluation of Endothelial Damage in Children with Crimean–Congo Hemorrhagic Fever PubMed: 25831422
Oncotarget Long noncoding RNA hotair mediated angiogenesis in nasopharyngeal carcinoma by direct and indirect signaling pathways Pubmed:26717040
Experimental Animals Ginsenoside Rb1 improves cardiac function and remodeling in heart failure. pubmed:28367863
Sci Rep.  Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. pubmed:28276491
PeerJ  The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker pubmed:28584715
Cellular Physiology and Biochemistry Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats pubmed:28848201
Tumor Biology Cervical cancer cell–derived angiopoietins promote tumor progression pubmed:28720059
Anesthesiology Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … Pubmed:28968277
British Journal of Anaesthesia Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats 10.1016:j.bja.2018.05.049
Anticancer Research Oligodendrocytes Up-regulate the Invasive Activity of Glioblastoma Cells via the Angiopoietin-2 Signaling Pathway Pubmed: 30711932
Comparative Medicine Characterization of a Porcine Model for Von Willebrand Disease Type 1 and 3 Regarding Expression of Angiogenic Mediators in the Nonpregnant Female … Pubmed: 31526432
BMC Endocrine Disorders The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children Pubmed: 31771561
Biology of Reproduction Expression of angiogenic factors in the uteroplacental unit is altered at time of placentation in a porcine model of von Willebrand disease type 1 Pubmed: 31806575
Scientific Reports Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea Pubmed: 31882827
MEDIATORS OF INFLAMMATION Mica Can Alleviate TNBS-Induced Colitis in Mice by Reducing Angiotensin II and IL-17A and Increasing Angiotensin-Converting Enzyme 2, Angiotensin 1-7 … Pubmed: 33100902
Scientific Reports Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats Pubmed: 32616735
Intensive Care Med Exp The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats 33997943
Gynecol Endocrinol Potential of thrombospondin-1 in treatment of polycystic ovary syndrome rat model: a preliminary study 34282706
Clinical and Experimental Immunology Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients Pubmed:35348620
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
RPA009Bo01 Recombinant Angiopoietin 2 (ANGPT2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA009Bo01 Polyclonal Antibody to Angiopoietin 2 (ANGPT2) WB; IHC; ICC; IP.
KSA009Bo01 ELISA Kit DIY Materials for Angiopoietin 2 (ANGPT2) Main materials for "Do It(ELISA Kit) Yourself"