Active Secondary Lymphoid Tissue Chemokine (SLC)
CCL21; 6Ckine; CKb9; ECL; SCYA21; TCA4; ECL; Chemokine(C-C-Motif)Ligand 21; Beta Chemokine Exodus-2; Efficient Chemoattractant For Lymphocytes
- Product No.APB575Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 97%
- Isoelectric Point10.0
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 211
For more details, please contact local distributors! US$ 528 US$ 1056 US$ 3168 US$ 7920
ACTIVITY TEST
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Secondary Lymphoid-tissue Chemokine (SLC) is a recently identified CC chemokine that is constitutively expressed in various lymphoid tissues and is a potent and specific chemoattractant for lymphocytes. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of SLC on the human T-lymphocyte leukemia cell line Jurkat. Briefly, Jurkat cells were seeded into the upper chambers (100uL cell suspension,106 cells/mL in RPMI 1640 with FBS free) and SLC (1ng/mL, 15ng/mL and 150ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows SLC is able to induce migration of Jurkat cells. The migrated Jurkat cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of SLC occurs at 15-150ng/mL.(A)Jurkat cells were seeded into the upper chambers and serum free RPMI 1640 with 150ng/mL SLC was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h;
(B)Jurkat cells were seeded into the upper chambers and serum free RPMI 1640 without SLC was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h.
Figure. The chemotactic effect of SLC on Jurkat cells
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Figure .The chemotactic effect of SLC on Jurkat cells.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine | Citations |
类风湿关节炎血清中CCL19 和CCL21 的表达水平与肺间质病变的关系 : | |
Behavioural Brain Research | Dysregulation of miR-142 results in anxiety-like behaviors following single prolonged stress Pubmed: 30857769 |
Neuroscience Letters | Sigma-1 receptor activation ameliorates LPS-induced NO production and ROS formation through the Nrf2/HO-1 signaling pathway in cultured astrocytes Pubmed: 31330223 |
Cell Mol Neurobiol | miR-132 Regulates PTSD-like Behaviors in Rats Following Single-Prolonged Stress Through Fragile X-Related Protein 1 Pubmed: 32333305 |
J Neuroinflammation | miR-142 downregulation alleviates rat PTSD-like behaviors, reduces the level of inflammatory cytokine expression and apoptosis in hippocampus, and?¡ 33407653 |
Exp Neurol | MiR-153 downregulation alleviates PTSD-like behaviors and reduces cell apoptosis by upregulating the Sigma-1 receptor in the hippocampus of rats exposed to … Pubmed:35259352 |