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Active Arginase (ARG)

ARG1; Arginase I; Liver Arginase

ACTIVITY TEST

Arginase (ARG) is a kind of inducible manganese (Mn2 )-containing enzymes that catalyse the final step of the urea cycle in the liver, converting l -arginine into l -ornithine and urea.Therefore, we can measure its bioactivity by enzyme activity assay. Recombinant human Arg was diluted to different concentrations in deionized water.Loading 40 µL of the rhARG1 and 10ul Substrate mixture(100 mM Arginine, 500 mM Glycine, 1.25 mM MnCl2, pH 10.5 ) into the plate. Incubated at 37 °C for 2 hours and stop the reaction with 200 µL stop solution of the 2 mM oPA, 2 mM NED ,50 mM Boric Acid, 1 M Sulfuric Acid, 0.03% Brij-35 (w/v) .Cover the plate and incubate at room temperature for 20 minutes and read at 520 nm (absorbance) in endpoint mode. The specific activity of recombinant human ARG is >24400pmol/min/ug.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
PLoS One Phenobarbital Induces Alterations in the Proteome of Hepatocytes and Mesenchymal Cells of Rat Livers Pubmed: 24204595
J Cereb Blood Flow Metab Arginase I release from activated neutrophils induces peripheral immunosuppression in a murine model of stroke PubMed: 25966956
Cancer Medicine Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease Pubmed:27109254
Cell Mol Immunol  Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496
Potent but transient immunosuppression of T-cells is a general feature of erythroid progenitor cells
Commun Biol Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells 34893694
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB120Hu01 Recombinant Arginase (ARG) Positive Control; Immunogen; SDS-PAGE; WB.
APB120Hu01 Active Arginase (ARG) Cell culture; Activity Assays.
PAB120Hu01 Polyclonal Antibody to Arginase (ARG) WB; IHC; ICC; IP.
LAB120Hu71 Biotin-Linked Polyclonal Antibody to Arginase (ARG) WB; IHC; ICC.
LAB120Hu81 FITC-Linked Polyclonal Antibody to Arginase (ARG) WB; IHC; ICC; IF.
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MAB120Hu23 Monoclonal Antibody to Arginase (ARG) WB; IHC; ICC/IF.
MAB120Hu24 Monoclonal Antibody to Arginase (ARG) WB; IHC; ICC; IP.
MAB120Hu21 Monoclonal Antibody to Arginase (ARG) WB; IHC
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SEB120Hu ELISA Kit for Arginase (ARG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB120Hu Multiplex Assay Kit for Arginase (ARG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB120Hu01 ELISA Kit DIY Materials for Arginase (ARG) Main materials for "Do It (ELISA Kit) Yourself".