Multiplex Assay Kit for Arginase (ARG) ,etc. by FLIA (Flow Luminescence Immunoassay)

ARG1; Arginase I; Liver Arginase

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Arginase (ARG) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Arginase (ARG) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Arginase (ARG) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Arginase (ARG) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 89-101 93
heparin plasma(n=5) 86-99 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginase (ARG) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginase (ARG) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Arginase (ARG) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-98% 94-105% 80-92% 82-91%
EDTA plasma(n=5) 90-97% 83-104% 97-104% 93-101%
heparin plasma(n=5) 84-98% 95-105% 93-104% 84-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:ARG) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
PLoS One Phenobarbital Induces Alterations in the Proteome of Hepatocytes and Mesenchymal Cells of Rat Livers Pubmed: 24204595
J Cereb Blood Flow Metab Arginase I release from activated neutrophils induces peripheral immunosuppression in a murine model of stroke PubMed: 25966956
Cancer Medicine Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease Pubmed:27109254
Cell Mol Immunol  Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496
Potent but transient immunosuppression of T-cells is a general feature of erythroid progenitor cells
Commun Biol Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells 34893694
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LAB120Hu71 Biotin-Linked Polyclonal Antibody to Arginase (ARG) WB; IHC; ICC.
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MAB120Hu24 Monoclonal Antibody to Arginase (ARG) WB; IHC; ICC; IP.
MAB120Hu21 Monoclonal Antibody to Arginase (ARG) WB; IHC
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KSB120Hu01 ELISA Kit DIY Materials for Arginase (ARG) Main materials for "Do It (ELISA Kit) Yourself".