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Active Fatty Acid Binding Protein 2, Intestinal (FABP2)

FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein

ACTIVITY TEST

Fatty Acid Binding Protein 2, Intestinal (FABP2) is a cytoplasmic protein highly expressed in enterocytes, responsible for intracellular trafficking of dietary long-chain fatty acids (FAs). It enhances FA uptake, solubilization, and transport to the endoplasmic reticulum for triglyceride synthesis and chylomicron assembly. FABP2 also modulates lipid metabolism by regulating peroxisome proliferator-activated receptor (PPAR) signaling. Genetic variants are linked to insulin resistance and cardiovascular risk due to altered FA metabolism.FABP2 can interact with APOA1 to promote the transfer of lipids to newborn high-density lipoprotein (HDL) particles, promote cholesterol efflux and HDL maturation.Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse FABP2 and recombinant human APOA1. Briefly, FABP2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to APOA1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FABP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse FABP2 and recombinant human APOA1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
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PLoS One Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats pubmed:28622357
Journal of Immunology and Immunopathology Effect of Porcine Rotavirus Enteritis on Concentrations of Interferonγ, Immunoglobulin-G and Intestinal Fatty Acid-Binding Protein-2 and Peripheral Leukocyte Function 10.5958/0973-9149.2017.00011.9
Cellular Physiology and Biochemistry Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory … Pubmed:29672286
European Journal of Clinical Nutrition Lack of liver steatosis in germ-free mice following hypercaloric diets Pubmed:29926176
Nutrients Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron Pubmed:29861488
International Surgery Journal Intestinal fatty acid binding protein (I-FABP) as a marker for acute intestinal ischemia Doi: 10.18203/2349-2902.isj20190058
Hepatology Communications Effect of a Multistrain Probiotic on Cognitive Function and Risk of Falls in Patients With Cirrhosis: A Randomized Trial
Tropical Animal Health and Production Immunomodulating dose of levamisole stimulates innate immune response and prevents intestinal damage in porcine rotavirus diarrhea: a restricted-randomized … Pubmed: 30790158
Biological & Pharmaceutical Bulletin Role of Aquaporin-3 in Intestinal Injury Induced by Sepsis Pubmed: 31582652
International Journal of Medical Sciences FABP1 and FABP2 as markers of diabetic nephropathy Pubmed: 32922200
Int Immunopharmacol Hydrogen attenuates radiation-induced intestinal damage by reducing oxidative stress and inflammatory response Pubmed: 32361189
Journal of Exercise Physiology Online Markers of Intestinal Damage in Individuals with and without Obesity during a 12-Week Exercise Period.
The Journal of Nutritional Biochemistry Effects of fucoidan on tumor prevention and gut flora
european journal of clinical nutrition? Effect of Lactobacillus casei on lipid metabolism and intestinal microflora in patients with alcoholic liver injury 33514869
J Cell Mol Med The ¦Á2AR/Caveolin©\1/p38MAPK/NF©\¦ÊB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia©\reperfusion 34114328
BMC Gastroenterol Somatization in patients with predominant diarrhoea irritable bowel syndrome: the role of the intestinal barrier function and integrity 34022802
JOURNAL OF APPLIED PHYSIOLOGY Impact of Successive Exertional Heat Injuries on Thermoregulatory and Systemic Inflammatory Responses in Mice 34528459
PLoS One The protective effects of HIF-1α activation on sepsis induced intestinal mucosal barrier injury in rats model of sepsis Pubmed:35576220
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