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Active Gelsolin (GSN)

GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor

ACTIVITY TEST

Gelsolin (GSN), one of the most abundant actin-binding proteins, is a multifunctional regulator of physiological and pathological cellular process and regulate cell migration, cell morphology, proliferation and apoptosis. Widely expressed, Gelsolin binds to actin and fibronectin, and is found both secreted in plasma and in cytoplasm and a previous study revealed that the levels of GSN are decreased in various cancer. Profilin-1 (PFN1) can also bind to actin and affects the structure of the cytoskeleton. PFN1 is one of the ligands of GSN, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GSN and recombinant human PFN1. Briefly, GSN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PFN1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GSN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human GSN and recombinant human PFN1 was shown in Figure 1, the EC50 for this effect is 1.11 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Neurochmical Research Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of Epilepsy SpringerLink: 3585r8h46q7r0171
Journal of Neuroinflammation Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury BioMed: 17422094
Biomedical Engineering and Informatics Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer IEEE: 6098563
European Journal of Obstetrics & Gynecology and Reproductive Biology Investigation of the actin scavenging system in pre-eclampsia Pubmed: 24239294
The Annals of Thoracic Surgery Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases Pubmed: 24565402
BMC Anesthesiol.? The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot study Pubmed:Pmc4132929
Cell Biochemistry and Biophysics Gelsolin: Role of a Functional Protein in Mitigating Radiation Injury Pubmed:25164111
Neurosci Lett Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer disease PubMed: 25864780
Proteomics Clinical Applications Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients Pubmed:26680509
PROTEOMICS - Clinical Applications Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. pubmed:26680509
Medicina Intensiva Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results. pubmed:28365030
Journal of Alzheimers Disease Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods Pubmed:30040720
World Journal of Pediatrics Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young children Pubmed:29427164
Biomedicines Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various … Pubmed:35327525
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA372Hu02 Recombinant Gelsolin (GSN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Hu05 Recombinant Gelsolin (GSN) Positive Control; Immunogen; SDS-PAGE; WB.
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RPA372Hu06 Recombinant Gelsolin (GSN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Hu03 Recombinant Gelsolin (GSN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Hu04 Recombinant Gelsolin (GSN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA372Hu01 Recombinant Gelsolin (GSN) Positive Control; Immunogen; SDS-PAGE; WB.
EPA372Hu61 Eukaryotic Gelsolin (GSN) Positive Control; Immunogen; SDS-PAGE; WB.
CPA372Hu21 OVA Conjugated Gelsolin (GSN) Immunogen; SDS-PAGE; WB.
PAA372Hu05 Polyclonal Antibody to Gelsolin (GSN) WB; IHC; ICC; IP.
PAA372Hu04 Polyclonal Antibody to Gelsolin (GSN) WB; IHC; ICC; IP.
PAA372Hu03 Polyclonal Antibody to Gelsolin (GSN) WB; IHC; ICC; IP.
PAA372Hu02 Polyclonal Antibody to Gelsolin (GSN) WB; IHC; ICC; IP.
PAA372Hu01 Polyclonal Antibody to Gelsolin (GSN) WB; IHC; ICC; IP.
PAA372Hu08 Polyclonal Antibody to Gelsolin (GSN) WB; IHC; ICC; IP.
LAA372Hu71 Biotin-Linked Polyclonal Antibody to Gelsolin (GSN) WB; IHC; ICC.
MAA372Hu27 Monoclonal Antibody to Gelsolin (GSN) WB; IHC; ICC; IP.
MAA372Hu29 Monoclonal Antibody to Gelsolin (GSN) WB; IHC; ICC; IP.
MAA372Hu22 Monoclonal Antibody to Gelsolin (GSN) WB; IHC; ICC; IP.
MAA372Hu24 Monoclonal Antibody to Gelsolin (GSN) WB; IHC
MAA372Hu21 Monoclonal Antibody to Gelsolin (GSN) WB; IHC; ICC; IP.
MAA372Hu23 Monoclonal Antibody to Gelsolin (GSN) WB; IHC; ICC; IP.
MAA372Hu25 Monoclonal Antibody to Gelsolin (GSN) WB; IHC; ICC; IP.
MAA372Hu26 Monoclonal Antibody to Gelsolin (GSN) WB; IHC; ICC; IP.
SEA372Hu ELISA Kit for Gelsolin (GSN) Enzyme-linked immunosorbent assay for Antigen Detection.
WEA372Hu Wide-range ELISA Kit for Gelsolin (GSN) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA372Hu CLIA Kit for Gelsolin (GSN) Chemiluminescent immunoassay for Antigen Detection.
LMA372Hu Multiplex Assay Kit for Gelsolin (GSN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA372Hu01 Antibody Pair for Gelsolin (GSN) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA372Hu01 ELISA Kit DIY Materials for Gelsolin (GSN) Main materials for "Do It (ELISA Kit) Yourself".