Active Gelsolin (GSN)
GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor
- Product No.APA372Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point5.9
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 80
For more details, please contact local distributors! US$ 200 US$ 400 US$ 1200 US$ 3000
ACTIVITY TEST
Gelsolin (GSN), one of the most abundant actin-binding proteins, is a multifunctional regulator of physiological and pathological cellular process and regulate cell migration, cell morphology, proliferation and apoptosis. Widely expressed, Gelsolin binds to actin and fibronectin, and is found both secreted in plasma and in cytoplasm and a previous study revealed that the levels of GSN are decreased in various cancer. Profilin-1 (PFN1) can also bind to actin and affects the structure of the cytoskeleton. PFN1 is one of the ligands of GSN, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GSN and recombinant human PFN1. Briefly, GSN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PFN1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GSN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human GSN and recombinant human PFN1 was shown in Figure 1, the EC50 for this effect is 1.11 ug/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
- BCA Protein Quantification Kit
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- Buffer
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- Spike RBD Protein (S-RBD)
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Magazine | Citations |
Neurochmical Research | Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of Epilepsy SpringerLink: 3585r8h46q7r0171 |
Journal of Neuroinflammation | Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury BioMed: 17422094 |
Biomedical Engineering and Informatics | Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer IEEE: 6098563 |
European Journal of Obstetrics & Gynecology and Reproductive Biology | Investigation of the actin scavenging system in pre-eclampsia Pubmed: 24239294 |
The Annals of Thoracic Surgery | Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases Pubmed: 24565402 |
BMC Anesthesiol.? | The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot study Pubmed:Pmc4132929 |
Cell Biochemistry and Biophysics | Gelsolin: Role of a Functional Protein in Mitigating Radiation Injury Pubmed:25164111 |
Neurosci Lett | Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer disease PubMed: 25864780 |
Proteomics Clinical Applications | Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients Pubmed:26680509 |
PROTEOMICS - Clinical Applications | Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. pubmed:26680509 |
Medicina Intensiva | Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results. pubmed:28365030 |
Journal of Alzheimers Disease | Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods Pubmed:30040720 |
World Journal of Pediatrics | Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young children Pubmed:29427164 |
Biomedicines | Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various … Pubmed:35327525 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPA372Hu02 | Recombinant Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA372Hu05 | Recombinant Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA372Hu61 | Active Gelsolin (GSN) | Cell culture; Activity Assays. |
RPA372Hu06 | Recombinant Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA372Hu03 | Recombinant Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA372Hu04 | Recombinant Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA372Hu01 | Recombinant Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA372Hu61 | Eukaryotic Gelsolin (GSN) | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA372Hu21 | OVA Conjugated Gelsolin (GSN) | Immunogen; SDS-PAGE; WB. |
PAA372Hu05 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
PAA372Hu04 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
PAA372Hu06 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
PAA372Hu03 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
PAA372Hu02 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
PAA372Hu01 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
PAA372Hu08 | Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
LAA372Hu71 | Biotin-Linked Polyclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC. |
MAA372Hu27 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
MAA372Hu29 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
MAA372Hu22 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
MAA372Hu24 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC |
MAA372Hu21 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
MAA372Hu23 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
MAA372Hu25 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
MAA372Hu26 | Monoclonal Antibody to Gelsolin (GSN) | WB; IHC; ICC; IP. |
SEA372Hu | ELISA Kit for Gelsolin (GSN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
WEA372Hu | Wide-range ELISA Kit for Gelsolin (GSN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA372Hu | CLIA Kit for Gelsolin (GSN) | Chemiluminescent immunoassay for Antigen Detection. |
LMA372Hu | Multiplex Assay Kit for Gelsolin (GSN) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
PSA372Hu01 | Antibody Pair for Gelsolin (GSN) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA372Hu01 | ELISA Kit DIY Materials for Gelsolin (GSN) | Main materials for "Do It (ELISA Kit) Yourself". |